His-Tag Purification

Introduction of His-Tag Purification

His-Tag is mainly composed of 6-10 consecutive histidine residues, which can be inserted at the C-terminal or N-terminal of the target protein. Most recombinant proteins are expressed using His-Tag tags, which interact specifically with metal ions, such as Ca²⁺, Mg²⁺, Ni²⁺, Cu²⁺, Fe³⁺, etc. As a result of its surface properties, His-tagged proteins can selectively bind to the chromatographic medium when they pass through a metal-ion-filled medium, whereas other impurity proteins cannot or only weakly bind. After buffer elution, a high-purity His-tagged protein is finally obtained.

Fig. 1 Schematic representation of basic cloning strategies and commonly used purification modalities. (David W W, 2014)

The Superiority of His-Tag Purification

• Tags are small and do not affect downstream proteins.
• As the His-Tag has an N-terminal location, it is consistent with the transcription and translation mechanisms of bacteria, facilitating protein expression.
• It has almost no effect on the properties of the target protein itself, and will not form dimers.
• Construct dual affinity tags with other affinity tags, and can be applied to a variety of expression systems.
• Using IMAC to purify His-Tag fusion protein, the operation is more convenient.
• With relatively low immunogenicity, the purified protein can be directly injected into animals for immunization and preparation of antibodies.

Advantages of Our Products

• High sequence compatibility
• Little effect of small sequence
• Low immunogenicity
• Strong joint usability
• Minimal impact on target expression and folding
• Wide range of applications and commercial systems
• Many commercial systems well established

Applications of Our Products

• Isolation and purification of recombinant proteins
• Detection of the target protein

Creative BioMart can provide you with a variety of high-quality His-Tag purification products for the purification and separation of recombinant proteins in a series of life science researches such as drug screening, cell biology research, and proteomics. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

Reference:

1. David W W. (2014) New trends and affinity tag designs for recombinant protein purification[J]. Current Opinion in Structural Biology. 26: 54-61.