Exosome Purification

Introduction of Extracellular Vesicles (EV)

Extracellular vesicles (EVs) are subcellular components secreted by cells, which can carry many signaling molecules and participate in many physiological and pathological activities of the body. The detection and analysis of EVs and their contents is conducive to the analysis of diseases from the cellular, subcellular, and molecular levels, and is of great value to the basic research and clinical application of disease-related mechanisms.

Exosomes are EVs with diameters ranging from approximately 40 to 160 nm that contain many components of cells such as DNA, RNA, lipids, metabolites, cytosolic, and cell surface proteins. Apart from their potential therapeutic value, exosomes can also be used to diagnose diseases.

Fig. 1 EV classification, biogenesis and isolation. (Carnino J M, et al., 2021)

Techniques for Isolation of EVs and Exosomes

• Differential ultracentrifugation
• Density gradient ultracentrifugation
• Precipitation-based
• SEC
• Immunoaffinity capture-based methods

Advantages of Our Products

• Ensure the structural and functional stability of purified vesicles
• Gentle non-invasive and inert method
• High product purity and less pollution
• High binding capacity
• High flow rate
• Strong rigidity
• Large stability

Applications of Our Products

• Separation of EVs and exosomes from various biological fluid samples such as whole blood, plasma, serum, and cell culture supernatant.
• Highly purified EVs and exosomes

Creative BioMart provides a series of products and tools for the extraction and isolation of EVs and exosomes, mainly including exorose beads and hydroxyapatite resin, which can help you further understand the function and structure of complex EVs and exosomes. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

Reference:

1. Carnino J M, Hao K Z, Jin Y. (2021) Extracellular vesicles: a novel opportunity for precision medicine in respiratory diseases[J]. Frontiers in medicine. 8: 661679.