Overview of Precast Protein Gel
Western Blot (WB) is one of the important methods for detecting proteins with antibodies, and the electrophoresis process is a key part of the experiment. Perfect electrophoresis can make the target bands neat and the resulting picture is eye-catching. The supporting medium of electrophoresis is polyacrylamide gel, and the polymerization of the gel is completed by N,N'-Methylenebisacrylamide and acrylamide monomers under the catalysis of free radicals.
| Traditional laboratory homemade gels compared to precast gels | |
|---|---|
| Traditional self-prepared gels | The shelf life of the separating gel is short and it will slowly hydrolyze after being left for a long time, so it can only be prepared and used immediately. |
| Acrylamide is a neurotoxic substance. When it is accidentally dropped on the skin, acrylamide can be absorbed through the skin and cause neurotoxicity and genotoxicity. | |
| If you want to pour gradient glue, you need to use a gradient mixer, which is not only complicated to operate but also cannot guarantee batch consistency. | |
| Precast protein gels | There is no need for manual pouring, and avoid contact with acrylamide-like toxic substances. |
| Different concentrations and gradients can be freely selected according to the condition of the protein sample. | |
| Due to the stable mass production process, the consistency of gel products can be guaranteed. | |
Features of Our Products
- High performance (strong separation ability and high resolution)
- Super long shelf life can be stored stably for one year
- Electrophoresis can be completed quickly
- High repeatability and good consistency of precast gels from different batches
- Compared with other brands in the market, high quality and low price can save experimental costs
Application Range of Our Products
- Western blotting
- Mass spectrometry
- Protein post-translational modifications
- Diluted samples
- Denaturing electrophoresis
According to different protein samples and electrophoresis application requirements, Creative BioMart provides precast gels with a complete range of concentrations and wells for you to choose from. Our Bis-Tris gel system provides a neutral pH environment that minimizes protein modifications, resulting in clearer and sharper bands without the typical "smiling" bands. Additionally, the system is important to maintain protein integrity when isolating low-abundance proteins. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.