Crosslinked Agarose Beads

Size exclusion chromatography (SEC) is one of the important techniques for studying protein purification. As the core of this technology, chromatography medium has always been a research hotspot. More and more new chromatographic mediums have been applied, such as artificially synthesized single-pore resins, agarose beads, and crosslinked polysaccharides with uniform particles. In addition to being easy to modify and activate, the crosslinked agarose bead has higher chemical and physical stability as well as less non-specific adsorption. Therefore, it is widely used in the SEC purification of downstream proteins, nucleic acids, and peptides in chemistry and bioengineering.

Fig. 1 Schematic overview of size exclusion chromatography (SEC). (Kummari R, et al., 2022)

Advantages of Our Products

• High chemical stability
• Fast flow rate
• Good mechanical properties
• Can be reused many times
• Improved ligand stability
• Efficient and industrial-scale purification
• High efficiency and simple operation
• High yield and high product purity
• High selectivity and excellent scalability
• Work in coordination with enzymes, proteins, and small antibodies

Applications of Our Products

• For batch or column purification
• Separation and purification of nucleic acids and proteins from cell extracts
• Antibody isolation from plasma
• Small-scale and large-scale purification of enzymes, native and recombinant proteins
• Isolate certain types of cells
• Separation, analysis, or characterization of chemicals and biochemicals
• Detection of biological interactions, including protein-protein, drug-protein, antibody-antigen, and receptor-ligand interactions

Creative BioMart has a wide range of high-quality crosslinked agarose beads for you to select for the successful purification of proteins and antibodies by SEC in the fields of biotechnology, microbiology, cell biology, and immunology. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

Reference:

1. Kummari R, Bose K. (2022) Gel Filtration Chromatography. In Textbook on Cloning, Expression and Purification of Recombinant Proteins[M]. Singapore: Springer Nature Singapore. 199-219.