Glyoxal Resins

Introduction of Glyoxal Resins

Affinity chromatography (AC) is a chromatographic technique designed using the specific affinity between biomolecules, which can quickly and efficiently separate biomacromolecules. By immobilizing one of the two molecules with affinity on an insoluble matrix, the other molecule is separated and purified by utilizing the specificity and reversibility of the intermolecular affinity.

Different matrices for AC include polyacrylamide, dextran/polystyrene, and agarose, among which agarose is the most widely used. Glyoxal resin allows the covalent attachment of agarose to lysine amino groups in the AC technique. The resin has a large number of aldehyde groups, which provides a very good choice for the conjugation of affinity ligands, thus improving the stability of the ligands.

Fig. 1 Schematic illustration of the affinity chromatographic purification of a target membrane protein

expressed in a bacterial host. (Hirschi S, et al., 2020)

Advantages of Our Products

• Improved ligand stability
• Efficient and industrial-scale purification
• High efficiency and simple operation
• High yield and high product purity
• High selectivity and excellent scalability
• Work in coordination with enzymes, proteins, and small antibodies

Applications of Our Products

• For batch or column purification
• Separation and purification of nucleic acids and proteins from cell extracts
• Antibody isolation from plasma
• Small-scale and large-scale purification of enzymes, native and recombinant proteins
• Isolate certain types of cells
• Separation, analysis, or characterization of chemicals and biochemicals
• Detection of biological interactions, including protein-protein, drug-protein, antibody-antigen, and receptor-ligand interactions

Creative BioMart has a wide range of high-quality glyoxal resins for you to select for the successful purification of proteins and antibodies by AC in the fields of biotechnology, microbiology, cell biology, and immunology. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Reference:

1. Hirschi S, Fotiadis D. (2020) Expression, Purification, and Structural Biology of Membrane Proteins[J]. Methods in Molecular Biology. 2127: 138-149.