Introduction of Protein Marker
In the process of western blot, the protein marker is used as a standard reference, which has a non-negligible effect on the experimental results. Its function is mainly to indicate the molecular weight corresponding to the protein band. Only when the standard amount is accurate can the experimental results be convincing. Further, the protein standard also serves as an indicator of whether or not the protein was transferred successfully or the degree to which it was electrophoresed on the gel, so selecting the appropriate protein marker is also an important aspect of western blot success.
Classification of Protein Marker
- Unprestained protein markers
- Broad molecular weight protein standard
- High molecular weight protein standard
- Low molecular weight protein standard
- Prestained protein markers
- Single-color pre-stain
- Multi-color pre-stain
Marker Selection Tips For You
| Tips For You | |
|---|---|
| Molecular weight range | Markers in the high molecular weight range are often used to detect large molecular weight proteins and markers in the small molecular weight range are often used to detect small proteins and even some polypeptides. If you consider the entire laboratory, choose a marker with a wide molecular weight and a relatively uniform band distribution, so that your protein can be easily judged no matter which interval it is in. |
| Strip accuracy | Non-prestained protein markers can be used as reference and calibration. |
| Stability | Whether the coupled dye is easy to fall off, making the color of the band lighter, and whether the marker is easy to degrade. |
| Affinity to membrane | Transferring to the membrane requires multiple washes and long-term incubation, so the affinity between the protein marker and the membrane is also very important. |
Advantages of Our Products
- Good consistency
- High accuracy
- Reasonable distribution of protein bands
- Low antibody hybridization
- Cost-effective
Applications of Our Products
- Polypropylene Gel Electrophoresis (SDS-PAGE)
- Western blot (WB)
- Isoelectric Focusing (IEF)
Creative BioMart can provide you with a variety of high-quality protein markers for a series of life science researches such as SDS-PAGE, western blot, and isoelectric focusing electrophoresis to facilitate real-time observation of the electrophoresis process and judgment of protein molecular weight. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.