MBP-Tag Purification

Introduction of MBP-Tag Purification

Maltose binding protein (MBP), which consists of 346 amino acid residues, has a molecular weight of about 42 kD. It is one of the members of the E. coli maltose transport system and is mainly responsible for the capture and transport of maltose. MBP can promote the soluble expression of fusion proteins, especially for eukaryotic proteins that are difficult to express, and membrane protein viral proteins have a good ability to solubilize and express. MBP-Tag is widely used due to the advantages of high expression level and easy purification of recombinant protein expressed by MBP fusion.

Fig 1. Chromatograph of a purification of an MBP-tagged fusion protein using amylose beads. (Duong-Ly K C, et al., 2015)

The Superiority of MBP-Tag Purification

• Simple affinity purification can be achieved
• Increase protein expression and protein stability
• Promote protein solubility and proper folding
• Reduce the degradation of the target protein
• Easy to scale up

Advantages of Our Products

• Can be re-used to purify the same protein
• High selectivity for high purity
• > 10 mg MBP tagged protein (80 kDa)/mL medium
• Stable coupling of ligand
• Low leakage

Applications of Our Products

• Isolation and purification of recombinant proteins
• Detection of the target protein

Creative BioMart can provide you with a variety of high-quality MBP-Tag purification products for the purification and separation of high-purity recombinant proteins in a series of life science researches such as drug screening, cell biology research, and proteomics. We offer Dextrin Agarose 6FF for the purification of proteins fused to MBP-tagged proteins by promoting proper folding of the attached protein while increasing the solubility of the target protein. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

Reference:

1. Duong-Ly K C, Gabelli S B. (2015) Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag. Methods in Enzymology. 559: 17–26.