Exosome Isolation Kit

Introduction of Exosome

Exosomes are nanoscale vesicles produced by the fusion of intracellular multivesicular bodies and cell membranes. They are widely distributed in almost all body fluids such as blood, urine, and pleural. Besides transferring genetic material and intercellular communication, exosomes also stimulate target cells by transferring bioactive molecules, which exert their biological functions. Researchers are primarily interested in using exosomes as therapeutic targets, drug carriers, or diseases.

Fig. 1 Extracellular vesicle (EV) biogenesis, subpopulations, and conventional and novel methods of exosome isolation. (Sidhom K, et al., 2020)

For subsequent research, it is crucial to obtain high-purity exosomes, and the isolation and purification of exosomes are always concerns of scientists.

Methods for Isolation and Purification of Exosomes

• Differential Ultracentrifugation (dUC)
• Ultrafiltration (UF)
• Poly-Ethylene Glycol (PEG)-Based Precipitation
• Immunoaffinity Capture
• Microfluidics
• Size-exclusion Chromatography (SEC)

Exosomes can be recovered from plasma morphologically and functionally intact by SEC, which is the best method for separating them from most proteins. However, this method takes a long time and is not suitable for processing a large number of samples.

Advantages of Our Products

• Easy to use
• No instrument required (no ultracentrifugation required)
• The extracted exosomes have a complete structure, are spherical or disk-shaped, and have uniform particles.
• The yield is high, and 3*10^11 exosome vesicles can be obtained in a single extraction.
• Suitable for the separation and extraction of exosomes in various cell supernatants

Applications of Our Products

• Separation of exosomes from various biological fluid samples such as whole blood, plasma, serum, and cell culture supernatant
• Highly purified exosomes

Creative BioMart provides high-quality exosome isolation kits for exosome extraction and isolation, which can help you understand the function and structure of complex exosomes and lay the foundation for further scientific research such as tumor cell migration, invasion, angiogenesis, and cell survival. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Reference:

1. Sidhom K, Obi P O, Saleem A. (2020) A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option? International Journal of Molecular Sciences. 21(18): 6466.