Spdtran

Gel filtration chromatography (GFC), also known as size exclusion chromatography (SEC), is a method that uses the network structure of the gel to separate molecules according to their size. The medium used for GFC is gel beads formed from cross-linked glucose, agarose, or polyacrylamide. Various types of agarose beads have different sieve sizes due to their porous inner network structure. Spdtran is a beaded gel filtration medium prepared by cross-linking dextran for the purification of protein and nucleic acid in biochemical experiments. Spdtran has high chemical stability and is widely used in the purification of downstream proteins, nucleic acids, and peptides in biopharmaceuticals and bioengineering.

Fig. 1 Illustration of the separation principle in gel filtration chromatography. (Kummari R, et ai., 2022)

Advantages of Our Products

• Good mechanical properties
• Can be reused many times
• Improved ligand stability
• Efficient and industrial-scale purification
• High efficiency and simple operation
• High yield and high product purity
• High selectivity and excellent scalability
• Work in coordination with enzymes, proteins, and small antibodies

Applications of Our Products

• For batch or column purification
• Separation and purification of nucleic acids and proteins from cell extracts
• Antibody isolation from plasma
• Small-scale and large-scale purification of enzymes, native and recombinant proteins
• Isolate certain types of cells
• Separation, analysis, or characterization of chemicals and biochemicals
• Detection of biological interactions, including protein-protein, drug-protein, antibody-antigen, and receptor-ligand interactions

Creative BioMart has a wide range of high-quality Spdtran gel filtration mediums in various pore sizes for you to select for the successful purification of proteins and antibodies by GFC in the fields of biotechnology, microbiology, cell biology, and immunology. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

Reference:

1. Kummari R, Bose K. (2022) Gel Filtration Chromatography. In Textbook on Cloning, Expression and Purification of Recombinant Proteins[M]. Singapore: Springer Nature Singapore. 199-219.