Stain

Introduction of Protein Stain

In biochemical experiments, the most important obstacle faced is obtaining high-purity and high-yield proteins. It is convenient for the experiment to be able to directly and rapidly detect and observe the quality change of the protein once it has been stained. By staining proteins separated on gels, key properties of these biopolymers, such as molecular weights and/or charges, can be determined. By utilizing staining, proteins can be detected on gels and blots. Different stains vary in color, sensitivity, stability, ease of use, specificity, and versatility. Commonly used protein staining reagents are divided into organic reagent staining represented by Coomassie brilliant blue(CBB), silver staining, fluorescent staining, and isotope color development.

Total Protein Staining Methods

• CBB Staining
• Colloidal Coomassie Staining
• Silver Staining
• Zinc Staining
• SYPRO

CBB and silver staining are frequently used in traditional protein staining, and the comparison of the two is as follows.

Fig1. Protein secretion of activated and non-activated S. scapterisci IJs. (Lu D, et al., 2017)

Advantages of Our Products

• High compatibility
• Safe and non-toxic
• High sensitivity
• Quick experiment
• Less variation between different proteins

Applications of Our Products

• Used for 2D gel analysis, vertical gel, and ordinary gel for determination of very low protein content.
• Used for staining of acid glycoproteins.
• Used for SDS-PAGE film staining, and can stain samples with a protein content of 10ng or more.
• Used for non-denaturing glue

Creative BioMart can provide a series of fast, simple, and high-quality protein stains to meet your scientific research needs. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

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Reference:

1. Lu D, Sepulveda C, Dillman AR. (2017) Infective Juveniles of the Entomopathogenic Nematode Steinernema scapterisci Are Preferentially Activated by Cricket Tissue. PLoS ONE. 12(1): e0169410.