Affinity Chromatography

Introduction of Affinity Chromatography

Affinity chromatography (AC) is a method for separating biomacromolecules by using the characteristics of specific recognition and reversible binding between biomacromolecules and some corresponding specific molecules. With this method, high-purity proteins are often obtained in one step, resulting in a well-established method for protein isolation. Proteins can be separated based on their specificity for a particular ligand rather than their ability to bind covalently. AC has been widely used in biotechnology fields such as serum separation, enzyme purification, antibody preparation, virus isolation, and genetic engineering. It can selectively isolate and enrich the biomacromolecules or cells needed for research, providing powerful technical support for many biological research and applications.

Fig. 1 The (a) sample application/washing steps and (b) examples of elution methods that are used in the on/off mode of affinity chromatography. (Rodriguez E L, et al., 2020)

Types of Our Products

• His-Tag Purification

His-Tag can be inserted into the C-terminal or N-terminal of the target protein, so most recombinant proteins are expressed by using it. Due to their surface properties, His-tagged proteins can be selectively combined with chromatographic analysis.

• GST-Tag Purification

GST can specifically bind to glutathione, acting as both an enzyme and a substrate. The GST tag is used to express the fusion protein, which specifically binds to the affinity medium with the glutathione ligand, thereby purifying the target protein.

• Biotin/Avidin Binding Purification

The biotin-avidin system (BAS) is a protein-ligand interaction system that exists in nature. This system has been successfully used in the detection and purification of proteins, nucleic acids, and lipids.

• Prepacked Cartridges

Easy one-step purification of immunoglobulins, especially IgG isotypes, and fusion proteins containing Fc regions.

• Immobilized-Metal AffinityChromatography (IMAC)

A commonly used protein purification technique is immobilized metal ion affinity chromatography (IMAC), which uses the interaction between metal ions and affinity groups in proteins separated from the mixture.

• MBP-Tag Purification

MBP can promote the soluble expression of fusion proteins, especially for eukaryotic proteins that are difficult to express, and membrane protein viral proteins have good solubility and expression capabilities.

• Other

Human albumin AC resin, heparin AC resin, hCG-AC resin, etc.

Advantages of Our Products

• Efficient and industrial-scale purification
• High efficiency and simple operation
• High yield and high product purity
• Non-mammalian-derived products reduce regulatory concerns
• High selectivity and excellent scalability

Applications of Our Products

• Separation and purification of nucleic acids and proteins from cell extracts
• Antibody Isolation from Plasma
• Small-scale and large-scale purification of enzymes, native and recombinant proteins
• Isolate certain types of cells
• Separation, analysis, or characterization of chemicals and biochemicals
• Detection of biological interactions, including protein-protein, drug-protein, antibody-antigen, and receptor-ligand interactions

Creative BioMart has a wide range of high-quality columns and resins for you to select for the successful purification of antibodies by AC in the fields of biotechnology, microbiology, cell biology, and immunology. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

Reference:

1. Rodriguez E L, Poddar S, Iftekhar S, et al. (2020) Affinity Chromatography: A Review of Trends and Developments over the Past 50 Years[J]. Journal of Chromatography B. 1157: 122332.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.