Biotin/Avidin Binding Purification

Introduction of Biotin/Avidin Binding

The biotin-avidin system (BAS) is a protein-ligand interaction system that exists in nature. This system has been successfully used in the detection and purification of proteins, nucleic acids, and lipids. BAS can be combined with affinity chromatography to greatly improve the purity of purified proteins, or to find receptors for known ligands. This method is widely used in the drug research and development industry. When a drug molecule has a therapeutic effect but it is not clear which protein it specifically acts on, it can be biotinylated and then the target protein can be captured from thousands of proteins.

Fig. 1 Capture coating schematic depicting the layers of the custom avidin-biotin complex adsorbed to optically polished, c-cut sapphire slide windows. (Trexler M, et al., 2021)

The Superiority of His-Tag Purification

• High sensitivity to improve the accuracy of the detection method
• High specificity due to the high affinity between avidin and biotin
• Universal and multifunctional
• High stability reduces operational errors and improves measurement reliability.
• Multi-stage signal amplification can maintain the original biological activity of macromolecular substances.

Advantages of Our Products

• High specificity
• High sensitivity
• Low cost
• The reaction time is short and the results are easy to observe.

Applications of Our Products

• Pull-down assays with biotinylated proteins
• Immunoprecipitation with biotinylated antibodies
• Purification of biotinylated molecules
• Antibodies
• Proteins
• Carbohydrates
• DNA

Creative BioMart can provide you with a variety of high-quality biotin/avidin binding purification products for the purification and separation of recombinant proteins in a series of life science researches such as drug screening, cell biology research, and proteomics. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

Reference:

1. Trexler M, Brusatori M, Auner G. (2021) Avidin-biotin complex-based capture coating platform for universal Influenza virus immobilization and characterization[J]. PLOS ONE. 16(2): e0247429.