Plain Agarose Beads

Introduction of Agarose Beads

The size of molecules in size exclusion chromatography (SEC) is determined by their apparent sizes, which depend on their molecular weight and shape. To enable higher flow rates and faster analysis, resins with solid cores and porous shells are commonly used in analytical SEC. Resins can be made from natural polymers (cross-linked dextran or agarose), synthetic polymers (cross-linked polyacrylamide or polymethacrylate), composites of natural and synthetic polymers, or silica. Agarose is an electrically neutral polysaccharide agar extracted from red algae. It has special gelling properties, especially remarkable stability, hysteresis, and hysteresis, and it is easy to absorb water and has a special stabilizing effect. With the development of biotechnology, it is more and more widely used in gel electrophoresis, affinity chromatography, molecular sieve, immunology research, and other fields.

Fig 1. SEC principle. (Hall M, 2018)

Advantages of Our Products

• High hydrophilicity and good heat resistance
• Excellent hydrophilicity
• Peak appear quickly
• High activity recovery rate
• High ion exchange capacity
• Accurate determination of molecular weight and molecular weight distribution
• High chemical stability and corrosion resistance
• The solute is independent between the stationary phase and the mobile phase without gradient elution.

Applications of Our Products

• Standard separation for quantifying protein dimers, trimers, and oligomers
• Calibration of complex polymers
• Biopharmaceutical characterization
• Routine monitoring of aggregation and quaternary structure of modified proteins (protein-drug conjugates and pegylated proteins)
• Guide the development of biopharmaceutical purification processes
• Changes in the extent and form of protein aggregation

SEC has been used for quality control of biological products since the early development of recombinant proteins for pharmaceutical use. Furthermore, the technology has been continuously improved and is now well-used for protein characterization.

Application of Our SEC Products

• Standard separations for quantifying protein dimers, trimers, and oligomers
• Calibration of complex polymers
• Biopharmaceutical characterization
• Routine monitoring of aggregation and quaternary structure of modified proteins (protein-drug conjugates and pegylated proteins)
• Guide the development of biopharmaceutical purification processes
• Changes in the extent and form of protein aggregation

Creative BioMart provides you with a series of fast, simple, and high-quality plain agarose beads for the separation, purification, and recovery of proteins, nucleic acids, bases, and carbohydrates in SEC, which can be used in bioengineering, pharmaceuticals, and food industries. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

Reference:

1. Hall M. (2018) Size Exclusion Chromatography (SEC). Biopharmaceutical Processing. 421–432.