Introduction of Affinity Chromatography
Affinity chromatography (AC), the most selective and versatile of the liquid chromatography methods, relies on proteins and their ligands such as hormones and their receptors, enzymes and their substrates, antibodies, and antigens, etc.) for reversible site-specific binding. Interactions can be used to immobilize a phase of interacting species on a column as a stationary phase for AC. The protein of interest is eluted from the complex fraction by selective adsorption of the affinity ligand. AC has become the most specific and effective chromatographic technique for separating and purifying biological macromolecules such as proteins and enzymes, and it has been widely used in biological separation.
Fig. 1 Affinity chromatography for separating biomolecules. (Silva J F, et al., 2023)
Types of Our Products
In the AC technique, glyoxal resins allow covalent attachment of agarose to lysine amino groups, providing a very good option for the conjugation of affinity ligands, resulting in improved ligand stability.
Through AC technology, aminoethyl resin has been widely used in the separation and purification of proteins in life science and chemical research.
Magnetic beads are versatile tools for the purification, detection, and quantification of analytes in complex matrices in biological experiments and scientific research.
Advantages of Our Products
- Efficient and industrial-scale purification
- High flexibility and reliability
- High efficiency and simple operation
- High yield and high product purity
- High selectivity and excellent scalability
- Quick response
Applications of Our Products
- Separation and purification of nucleic acids and proteins from cell extracts
- Antibody isolation from plasma
- Small-scale and large-scale purification of enzymes, native and recombinant proteins
- Isolate certain types of cells
- Separation, analysis, or characterization of chemicals and biochemicals
- Detection of biological interactions, including protein-protein, drug-protein, antibody-antigen, and receptor-ligand interactions
- Immunocapture, cell-stimulating, biopanning, and flow cytometry
Creative BioMart can provide you with various fast, efficient, and selective affinity coupling products (resin and magnetic beads) for the purification and separation of proteins with AC technology in the fields of biotechnology, microbiology, cell biology, and immunology. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.
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Reference:
- Silva J F, Lima C M G, Silva D L, et al. (2023) Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents. Separations. 10(1): 36.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.