Ceramic Hydroxyapatite Resin

Introduction of Ceramic Hydroxyapatite

Ceramic hydroxyapatite (CHT) chromatography is a common separation technique that can be used to separate and purify biomacromolecules such as proteins and nucleic acids. By utilizing the special properties of CHT, the target molecules are separated from other impurity molecules. By exchanging cations between phosphate and calcium ions, CHT achieves the purpose of eluting protein. The removal of polymers, antibody fragments, viruses, nucleic acids, and other impurities in the antibody production process can be effectively achieved by CHT, especially the removal of high polymers. CHT has become a key technology for the purification of many antibodies, vaccines, and other drugs, and has broad application prospects in the fields of biomedicine, bioengineering, and biopharmaceuticals in the future.

Fig 1. The mechanism of CHT resin interaction with rAAV-KAL in the presence of Ca²⁺. (Qu Weihong, et al., 2015)

Advantages of Our Products

• High separation efficiency and resolution
• Excellent capture at elevated flow rates
• Large capacity for high-titer upstream feedstocks
• Exceptional selectivity for the two-step chromatographic process
• High-density particles for fast and simple column packing
• Rigid particles for fast cleaning and equilibration
• Inorganic calcium phosphate for distinct selectivity

Applications of Our Products

• Antibody of different subtypes and polyclonal antibody
• Basic proteins (immunoglobulins), recombinant proteins, and complex protein mixtures
• Plasmid
• Single-stranded DNA and double-stranded DNA
• Endotoxin

Creative BioMart has a wide range of high-quality columns and resins for you to select for the successful purification of proteins and nucleic acids by CHT chromatography. CHT resins we provide can be used to separate host proteins (HCP), shed protein A, antibody dimers and aggregates, nucleic acids, and viruses, as well as excel at every stage of capture, intermediate purification, and purification. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

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Reference:

1. Qu Weihong, Wang Mingxi, Wu Yaqing, et al. (2015) Calcium-ion-modulated ceramic hydroxyapatite resin for the scalable purification of recombinant Adeno-Associated Virus serotype 9[J]. Journal of Chromatography B. 990: 15-22.