| Cat# | Product Name | Size | Price | Qty | Inquiry |
|---|---|---|---|---|---|
| Hi-081P | HiTrap Streptavidin HP | Inquiry |
| Product Information | |
| Cat#No# | Hi-081P |
| Product Overview | HiTrap Streptavidin HP is a column prepacked with Streptavidin Sepharose High Performance for purification of biotinylated proteins and biomolecules. |
| Description | Streptavidin Sepharose High Performance is an affinity chromatography medium designed for fast and reliable binding of biotinylated substances. The medium is very useful since any biotinylated substance bound via streptavidin can be used as a ligand for an affinity purification. Prepacked, ready to use, HiTrap columns offer added convenience and speed. Streptavidin Sepharose High Performance is also supplied as a preswollen medium in suspension. |
| Characteristic | Convenient, fast, reliable purification of biotinylated compounds. The strong interactions of biotin and streptavidin, as well as the somewhat weaker interactions of 2-iminobiotin and streptavidin are utilized. The column can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System. |
| Maximum operating pressure | 5 bar [0.5 MPa] (70 psi) |
| Sample preparation | The sample should be adjusted to the composition of the binding buffer. This can be done either by diluting the sample with binding buffer or by buffer exchange using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 Desalting columns. The sample should be filtered through a 0.45 μm filter or centrifuged immediately before it is applied to the column. |
| Matrix | Highly cross-linked spherical agarose. |
| Average particle size | 34 µm |
| Ligand | Streptavidin |
| Dynamic binding capacity | Biotin, > 300 nmol/ml medium Biotinylated bovine serum albumin, 6 mg/ml medium. |
| Recommended flow rate | < 4 ml/min |
| Recommended column height | 25 mm |
| pH working range | 2 to 10.5 |
| CIP stability | 4 to 9 |
| Temperature stability | 4°C to room temperature |
| Storage | 4 to 30°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8. |
| Pack size | 5 × 1 mL |
| Maximum flow velocity | 4 ml/min |
| Dimensions | 7 × 25 mm |
| Column volume | 1 mL |
| Column i.d. | 7 mm |
| Column hardware pressure limit | 5 bar (0.5 MPa) |
Download Datasheet
|
Download SDS
|
For Research or Industrial Raw Materials, Not For Personal Medical Use!Online Inquiry
