StrepTrap XT

• Cat#No#: XT-118P
• Product Overview: A chromatography resin for the purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag, which provides higher affinity, resulting in higher yields and lower loss of valuable target protein.
• Characteristic: Highly pure Strep-tag II and Twin-Strep-tag recombinant proteins.
  Fast purifications with simple elution.
  Physiological conditions and mild elution preserve target protein activity.
  Fast and easy regeneration.
  Compatible with a wide range of detergents, denaturants, and other additives.
  Available in 10 and 50 mL lab packs of bulk resin and prepacked in 1 and 5 mL StrepTrap XT chromatography columns for convenience.
• Applications: Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag.
• Sample preparation: Adjust the sample to the composition of the binding buffer. Either dilute the sample with binding buffer or exchange buffer using a column for desalting.
  To avoid clogging the column, clarify the sample by centrifugation and filtration through a 0.45 μm filter immediately before application.
• Matrix: Rigid highly cross-linked 6% agarose
• Average particle size: 34 μm
• Ligand: Strep-Tactin XT
• Ligand density: Approx. 5 mg/mL resin
• Dynamic binding capacity: Approx. 10 mg protein with Strep-tag II or Twin-Strep-tag/mL resin.
• Recommended flow rate: 1 mL/min
• Recommended column height: 25 mm
• Chemical stability: Stable in all commonly used aqueous buffers.
• pH working range: 6 to 10
• Storage: 2°C to 8°C in 20% ethanol
• Cleaning-in-place: 6 M urea or 4 M GuHCl can also be used to remove non-specifically bound proteins.
• Pack size: 1 × 1 mL
• Maximum flow velocity: 4 and 20 mL/min for 1 and 5 mL columns respectively.
• Dimensions: 7 × 25 mm
• Column volume: 1 ml
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)