| Product Information | |
| Cat#No# | PPC-005 |
| Product Overview | The iCapTag resin is designed to capture recombinant proteins tagged with the iCapTag, a self-cleaving affinity tag for the rapid and simple purification of tagless proteins. Prepacked iCapTag columns are designed for simple purification of tagless proteins via a self-cleaving affinity tag. |
| Cleavability | 95% or higher cleavage in 5 hours at room temperature (based on GFP test target protein) |
| Base Bead | Hardened agarose (6% cross-linked agarose) |
| Ligand Density | 15 ± 2 g/L |
| Average Particle Size | ~90 μm |
| Recommended Maximum Operating Flow Velocity | 300 cm/h in a 5 cm diameter x 10 cm bed height column using aqueous buffer |
| Maximum Back Pressure | 40 psi (2.8 bar; 0.28 MPa) |
| Chemical Stability | Resin is initially stripped at pH ≤ 2.0 followed by buffers with the pH between 6.2 and 8.5. Resin is neutral to salts, sugars and other buffer additives, including protease inhibitors and stays active when caustic buffers are used. |
| Resin Packing | 50% slurry in storage solvent in 18% ± 2% ethanol |
| Temperature Stability | 4 °C to 36 °C |
| Storage | 18% ± 2% ethanol, 4°C to 8°C |
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