This matrix is a highly crosslinked 4% agarose resin with a bead size of 50-150 µm. It is a gel filtration resin developed for industrial-scale separation of large molecules utilizing higher pressure and flow rates than standard sized beads. The matrix is not pre-activated so the user needs to generate groups for coupling procedures.
Form
20% Ethanol
Matrix
Highly crosslinked
Molecular Characterization
Exclusion Limit Da: ~3×10^7
Average particle size
Spherical,Standard: ~ 50-150 µm
Recommended flow rate
Maximum Flow Rate at 15 cm Bed Height ≥ 500 cm/h
Maximum Pressure Drop
Maximum Pressure at 15 cm bed Height ≥150 kPa
Chemical stability
Most commonly used aqueous and organic solutions including: 1M NaOH, 8M Urea, 6M guanidine HCl, 75% ethanol
pH working range
pH Stability: pH 1.8-14 short term / pH 3.8-13 long term