Ab buffer kit

• Cat#No#: Ab-324C
• Product Overview: Ab Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols. The kit also includes neutralizing buffer. Includes: 1 × 50 ml Binding buffer 10× stock solution, 1 × 15 ml Elution buffer 10× stock solution, and 1 × 25 ml Neutralizing buffer.
• Description: Ab Buffer Kit contains 10X stock solutions of Binding and Elution buffers and ready to use Neutralizing buffer. The buffers are optimized for rapid purification of monoclonal and polyclonal IgG using immobilized Protein A or Protein G. The kit eliminates timeconsuming buffer preparation and thus promotes fast, reproducible and convenient purification work. The buffers have been prepared using the highest quality chemicals and water, and have been filtered through a 0.45μm filter.
• Characteristic: High purity and yield of antibodies.
  Simple and proven method giving reproducible results.
  Short purification time due to the prepacked format and no sample pre-treatment.
• Applications: 600μL (to be able to compare the results, the sample was diluted 1:2 with Binding buffer and filtered through 0.2μm).
• Sample preparation: The concentration of antibodies varies depending on source used in the production. For cell culture, the specific monoclonal antibody concentration is in the range of 0.05–1 mg/mL.
  If the monoclonal antibody is present at a low concentration, multiple loading might be needed. The antibody can then be concentrated and eluted in small volumes. Three repeated sample loading (3 × 600 μL) of a cell culture supernatant containing a mouse IgG2a was performed on two Ab SpinTrap columns. The eluted fractions were pooled and analyzed by SDS-PAGE. Results show that the enriched eluted antibody is very pure (> 90%).
• Medium Preparation: Protein G Sepharose High Performance
• Column: Polypropylene barrel, polyethylene frits
• Average particle size: 34 μm
• Dynamic binding capacity: > 1 mg IgG/column
• Chemical compatibility: Stable in all buffers commonly used in antibody purification
• pH working range: 2–9
• Storage: 4–8°C, 20% Ethanol
• Pack size: 1
• BioProcess resin: 100 μL