Product Information | |
Product Overview | Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by boiling in SDS Page Sample Loading Buffer. Avidin is a glycoprotein with approximately 10% of its total mass coming from carbohydrates. Avidin has a molecular weight of 67kDa and contains four identical 128 amino acid subunits that each has a single biotin binding domain. Avidin is a basic protein with an isoelectric pH of 10-10.5 and is readily soluble in aqueous buffers containing a wide range of salt, pH (2-11), temperature and other laboratory agents. This wide range of tolerance makes avidin suitable for a wide variety of analytical applications. Avidin has extraordinary binding affinity for biotin (Ka=1015M-1). The avidin in covalently coupled to the agarose resulting in minimal leaching and is stable over pH2-11. The Avidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. The resin can also be used for immunoprecipitations using biotin labeled antibodies. Supplied as a 50% resin slurry. |
Optimum pH | 2-11 |
Characteristic | Avidin covalently coupled to ~6% cross linked agarose. Minimal Leaching Binding capacity 15-20µg biotin/ml resin Bead Structure: 6% cross-linked agarose |
Applications | Immunoprecipitation with biotinylated antibodies Pull down assays with biotinylated proteins Purification of biotinylated molecules, including: Proteins Antibodies DNA Carbohydrates |
Storage | It is shipped at ambient temperature. Upon arrival, store refrigerated at 4°C, DO NOT FREEZE. This product is stable for 1 year at 4°C |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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