Albumin and IgG Depletion SpinTrap

• Cat#No#: Al-001P
• Product Overview: Albumin and IgG Depletion SpinTrap are prepacked columns for the depletion of albumin and IgG from human serum and plasma. They are prepacked with high performance Sepharose based resins with an affinity for human serum albumin (HSA) and IgG.
• Description: HiTrap Albumin and IgG Depletion and Albumin and IgG Depletion SpinTrap are prepacked columns for the depletion of albumin and IgG from human serum and plasma. The columns are prepacked with high performance Sepharose based media with an affinity for human serum albumin (HSA) and IgG. These columns are members of the Trap platform and address the need for flexible, small-scale preparation of protein samples prior to downstream analyses such as 1-D or 2-D gel electrophoresis and mass spectrometry.
  A common problem when studying plasma or serum is the difficulty in detecting less abundant proteins. The most abundant plasma proteins are albumin and IgG, and they tend to obscure the signals of less abundant proteins. The high abundance of albumin and IgG also interferes with the detection of other proteins by preventing a sufficient amount of less abundant proteins from being included in the analysis. By depleting samples of albumin and IgG, the quality and depth of the analysis can be greatly enhanced. Depletion of the two proteins removes more than 60% of the total protein content in human plasma, allowing proteins normally obscured by albumin and IgG to be visualized.
• Characteristic: Albumin and IgG Depletion SpinTrap is a prepacked column for the depletion of albumin and IgG from human serum or plasma.
  Albumin and IgG Depletion SpinTrap handles smaller sample volumes (~50 μl) using a tabletop centrifuge.
  Columns are prepacked with high performance Sepharose based resin with an affinity for human serum albumin (HSA) and IgG.
  High depletion capacity, removes > 95% HSA and > 90% IgG, with high reproducibility.
  Simple and fast procedure.
  Removal of albumin and IgG allows a higher load of less abundant proteins to be included in the analysis and detection of more proteins.
• Matrix: Highly cross-linked 6% agarose
• Average particle size: 34 µm
• Ligand: Recombinant Protein G fragment and recombinant protein binding HAS.
• Recommended flow rate: 1 ml/min
• pH working range: 2 to 9
• CIP stability: 3 to 9
• Shelf life: 24 months
• Storage: 2 to 8°C, in 20% Ethanol
• Pack size: 10 columns
• Maximum flow velocity: 4 ml/min
• Dimensions: 0.7 × 2.5 cm
• Column volume: 1 ml
• Column hardware pressure limit: 5 bar (0.5 MPa, 70 psi)