Alkali-Tolerant Protein A Agarose 4 Fast Flow

• Cat#No#: AC-014A
• Common Name: Protein A
• Product Overview: rProtein A Agarose 4 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes.
  Alkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 4% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.
• Matrix: Cross-linked 4% agarose supplied as a 50% slurry
• Average particle size: 45 μm - 165 μm, with an average of ~ 90 μm
• Ligand: Recombinant alkali-tolerant protein A produced in E.coli
• Dynamic binding capacity: >40 mg human IgG/mL medium
• Recommended flow rate: Gravity flow and medium pressure, 30 - 300 cm/hour
• Maximum Pressure Drop: 0.3 MPa (3 bar)
• Chemical stability: All commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.
• CIP stability: 0.1 M ~ 0.5 M NaOH
• Storage: 2 - 8°C, storage buffer: 20% ethanol