Cat# | Product Name | Size | Price | Qty | Inquiry |
---|---|---|---|---|---|
AC-020A | Alkali-Tolerant Protein A Agarose 4FF, Prepacked Cartridge | 5x1ml | Inquiry | ||
1x5ml | Inquiry | ||||
5x5ml | Inquiry |
Product Information | |
Cat#No# | AC-020A |
Common Name | Protein A |
Product Overview | rProtein A Agarose 4 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes. Alkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 4% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low. |
Matrix | Cross-linked 4% agarose |
Average particle size | 45 μm - 165 μm, with an average size of ~ 90 μm |
Ligand | Recombinant alkali-tolerant protein A produced in E.coli |
Dynamic binding capacity | >40 mg human IgG/mL medium |
Recommended flow rate | Gravity flow and medium pressure, 30 - 300 cm/hour |
Maximum Pressure Drop | 0.3 MPa (3 bar) |
Chemical stability | All commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea. |
CIP stability | 0.1 M ~ 0.5 M NaOH |
Storage | 2 - 8°C, storage buffer: 20% ethanol |
Download Datasheet | Download SDS |
For Research or Industrial Raw Materials, Not For Personal Medical Use!
Online Inquiry