Alpha-Chymotrypsin is a serine endoprotease that preferentially targets the C-terminal peptide bonds of large hydrophobic residues, including Tyr, Phe, Trp, and Leu. Secondary cleavage may occur at Met, Ala, Asp, and Glu. During production, TLCK is utilized to neutralize residual trypsin activity, while calcium ions are added to enhance the enzyme's stability and activation.
Physical Appearance
Lyophilized powder
Molecular Weight
25 kDa
Resuspension Buffer
Recommended: Dissolve the powder in 100 μL of 1 mM HCl supplemented with 2 μM CaCl2.
Storage
Lyophilized powder: -20 °C storage; dissolved enzyme: -20 °C (shelf life of 7 days).
Shelf life
Store at -80 ℃ for up to 24 months.
Stability
Optimal catalytic efficiency is achieved at pH 7.8.
Usage Notes
Apply α-chymotrypsin at a 1:50 (w/w) protease-to-protein loading. The digestion should proceed in a 100 mM Tris-HCl buffer (pH 7.8) supplemented with 10 mM CaCl2. Incubate at 30 °C for a duration of 2 to 12 hours.
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For Research or Industrial Raw Materials, Not For Personal Medical Use!