ANX Sepharose 4 Fast Flow (High Sub)

• Cat#No#: AN-436C
• Product Overview: ANX Sepharose 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers. It is optimized for the separation of larger proteins.
  Weak anion exchanger with different selectivity compared with traditional ion exchangers.
  Applicable for separation of high molecular weight proteins.
  Developed in co-operation with leading large-scale pharmaceutical manufacturers.
  High chemical stability allows for well proven CIP and sanitization protocols.
  The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
• Description: ANX Sepharose Fast Flow (high sub) is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. ANX Sepharose 4 Fast Flow (high sub) is a weak anion exchanger resin with tertiary amine groups attached to the base matrix Sepharose 4 Fast Flow via ether linkage and a hydrophilic spacer arm. The diethylaminopropylgroup is coupled in a mode that precludes formation of quaternary groups, which are typically seen on traditional DEAE media, resulting in a truly weak anion exchanger. The base matrix, Sepharose 4 Fast Flow, is a highly cross-linked, 4% agarose derivative with high chemical and physical stability and broad separation range.
• Characteristic: High binding capacity and good flow properties.
  High chemical and physical stabilities.
  Reliable and reproducible performance.
  Easy and effective cleaning-in-place (CIP)/sanitization.
  Various, convenient prepacked column formats.
  Predictable scale-up.
• Maximum operating pressure: ≥200 cm/h at <0.1 MPa in a XK 50/60 column.
• Ligand Coupling Method: Ether linkages and hydrophilic spacer arm
• Metal ion capacity: 0.13-0.18 mmol Cl-/ml medium
• Matrix: cross-linked agarose, 4%, spherical
• Ionic Exchanger Type: Weak anion exchanger
• Average particle size: ~90 µm
• Ligand: Diethylaminopropyl
• Recommended column height: 25 cm
• Chemical stability: Stable in commonly used aqueous buffers: 1.0 M NaOH, 20% and 70% ethanol, 8 M urea, 8 M guanidine HCl, 1M acetic acid, aqueous buffers -1.0 M NaOH, - 20 and 70% Ethanol.
• pH working range: 3–13
• CIP stability: 2–14
• Storage: 4 to 30°C, 20% Ethanol
• Pack size: 25 mL
• BioProcess resin: Yes
• Dimensions: 5 cm