Benzamidine Sepharose 4 Fast Flow (High Sub)

• Cat#No#: Be-344C
• Product Overview: Benzamidine Sepharose 4 Fast Flow (High Sub) is an excellent tool for removal of proteolytic activity from a protein.
  Benzamidine Sepharose 4 Fast Flow (high sub) is based on highly cross-linked 4% agarose enabling high flow rates without losing binding capacity.
  p-aminobenzamidine (pABA) immobilized on Sepharose 4 Fast Flow matrix gives high productivity, and allows easy scale-up.
  Effective removal of thrombin and factor Xa after tag cleavage of recombinant proteins.
  For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
• Description: Benzamidine Sepharose 4 Fast Flow (high sub) is an excellent tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as, zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
• Characteristic: Benzamidine Sepharose 4 Fast Flow (high sub) is based on highly crosslinked 4% agarose, which enables rapid processing of large sample volumes. The ligand, paminobenzamidine, is covalently coupled via an amide bond to a long spacer arm attached to Sepharose 4 Fast Flow via a stable ether linkage. Coupling is optimized to give high binding capacity. Total binding capacity is ≥ 35 mg trypsin per mL medium.
• Maximum operating pressure: base matrix min 150 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm.
• Ligand Coupling Method: Amide
• Packing Column: Benzamidine Sepharose 4 Fast Flow (high sub) is supplied in 0.05 M acetate buffer pH 4 containing 20% ethanol as a bacteriostat. Decant the solution and replace it with binding buffer before use. In general, we recommend a bed height of 5–15 cm to allow high flow rates to be used.
• Matrix: Highly cross-linked 4% agarose
• Average particle size: ~90 µm
• Ligand: p-aminobenzamidine [pABA]
• Ligand density: > 12 µmol p-aminobenzamidine/ml medium
• Recommended flow rate: 30 cm/h - 300 cm/h
• Recommended column height: 25 cm
• Chemical stability: Commonly used aqueous buffers
• pH working range: 2–8
• CIP stability: 1–9
• Storage: 2 to 8°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol
• Shipping: Supplied in 0.05 M acetate buffer, pH 4 containing 20% ethanol.
• Cleaning-in-place: The general recommendation is to use a solution of guanidine hydrochloride to remove precipitated or denatured substances. For hydrophobically bound substances, a solution of non-ionic detergent or ethanol is recommended.
• Sanitization: For sanitization of Benzamidine Sepharose 4 Fast Flow, we recommend storage in a solution containing 0.1 M acetic acid/20% ethanol.
• Pack size: 100 mL
• Maximum flow velocity: 300 cm/h