Benzamidine Sepharose 4 Fast Flow (Low Sub)

• Cat#No#: Be-345C
• Product Overview: Benzamidine Sepharose 4 Fast Flow (low sub) is an affinity medium based on a highly cross-linked 4% agarose matrix, which enables rapid processing of large sample volumes. p-aminobenzamidine, a synthetic inhibitor of trypsin and trypsin-like serine proteases, is covalently bound to a long spacer arm attached to Sepharose 4 Fast Flow via a stable ether linkage. Benzamidine Sepharose 4 Fast Flow (low sub) has been used for purification of several enzymes such as trypsin and enterokinase.
• Characteristic: Ideal for industrial purification of serine proteases.
  Selectivity similar to Benzamidine Sepharose 6B, but is more suitable for large-scale applications due to the Sepharose Fast Flow matrix.
  Long spacer arm makes the small ligand easily available for binding target molecules.
• Maximum operating pressure: Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Ligand Coupling Method: Amide linkage
• Matrix: 4% cross-linked agarose
• Particle Size: 45 μm-165 μm
• Average particle size: ~90 µm
• Ligand: p-aminobenzamidine
• Ligand density: Aprox. 6 μmol/ml
• Coupling chemistry: Amide linkage (carbodiimide)
• Dynamic binding capacity: 25 mg Trypsin / mL
• Recommended flow rate: Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Recommended column height: 25 cm
• Chemical stability: Stable to commonly used aqueous buffers: 7.2 M guanidine hydrochloride.
• pH working range: 2–8
• CIP stability: 1–9
• Storage: 4 to 8°C, in 20% Ethanol and 0.05 M Sodium Acetate
• Cleaning-in-place: The general recommendation is to use a solution of guanidine hydrochloride to remove precipitated or denatured substances. For hydrophobically bound substances, a solution of non-ionic detergent or ethanol is recommended.
• Sanitization: For sanitization of Benzamidine Sepharose 4 Fast Flow, we recommend storage in a solution containing 0.1 M acetic acid/20% ethanol.
• Pack size: 100 mL
• BioProcess resin: No
• Maximum flow velocity: 250 cm/h