| Product Information | |
| Cat#No# | Be-345C |
| Product Overview | Benzamidine Sepharose 4 Fast Flow (low sub) is an affinity medium based on a highly cross-linked 4% agarose matrix, which enables rapid processing of large sample volumes. p-aminobenzamidine, a synthetic inhibitor of trypsin and trypsin-like serine proteases, is covalently bound to a long spacer arm attached to Sepharose 4 Fast Flow via a stable ether linkage. Benzamidine Sepharose 4 Fast Flow (low sub) has been used for purification of several enzymes such as trypsin and enterokinase. |
| Characteristic | Ideal for industrial purification of serine proteases. Selectivity similar to Benzamidine Sepharose 6B, but is more suitable for large-scale applications due to the Sepharose Fast Flow matrix. Long spacer arm makes the small ligand easily available for binding target molecules. |
| Maximum operating pressure | Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
| Ligand Coupling Method | Amide linkage |
| Matrix | 4% cross-linked agarose |
| Particle Size | 45 μm-165 μm |
| Average particle size | ~90 µm |
| Ligand | p-aminobenzamidine |
| Ligand density | Aprox. 6 μmol/ml |
| Coupling chemistry | Amide linkage (carbodiimide) |
| Dynamic binding capacity | 25 mg Trypsin / mL |
| Recommended flow rate | Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
| Recommended column height | 25 cm |
| Chemical stability | Stable to commonly used aqueous buffers: 7.2 M guanidine hydrochloride. |
| pH working range | 2–8 |
| CIP stability | 1–9 |
| Storage | 4 to 8°C, in 20% Ethanol and 0.05 M Sodium Acetate |
| Cleaning-in-place | The general recommendation is to use a solution of guanidine hydrochloride to remove precipitated or denatured substances. For hydrophobically bound substances, a solution of non-ionic detergent or ethanol is recommended. |
| Sanitization | For sanitization of Benzamidine Sepharose 4 Fast Flow, we recommend storage in a solution containing 0.1 M acetic acid/20% ethanol. |
| Pack size | 100 mL |
| BioProcess resin | No |
| Maximum flow velocity | 250 cm/h |
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