This affinity resin is physically and chemically stable, has durable ligands, long lifetime, and broad applicability. It binds proteins via specific and non-specific interactions (charge, hydrophobic). It is widely used for purification of dehydrogenases, kinases, transferases, serum albumin, interferons, and plasma proteins.
Matrix
6% Highly cross-linked agarose
Appearance
Blue paste, may separate upon standing
Wet Particle Size Range
45–165 μm
Ligand
Cibacron blue 3GA
Dynamic binding capacity
> 18 mg HSA / mL packed resin
Coupling chemistry
Triazine chemistry with cibacron blue 3GA
pH working range
4–12
pH CIP range
3–13
Chemical stability
Stable in common aqueous solutions: 8 M urea, 6 M guanidine HCl, 70% ethanol
Maximum operating pressure
0.3 MPa
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L
Storage
2–8 °C in 20% ethanol or 2% benzyl alcohol, 0.1 M KH2PO4, pH 8.0
Product features
Rapid, efficient large-scale processing, high dynamic binding capacity, high elution yield, physically and chemically stable.