Butyl-S Sepharose 6 Fast Flow

• Cat#No#: Bu-414C
• Product Overview: Butyl-S Sepharose 6 Fast Flow is the least hydrophobic resin in the Sepharose Fast Flow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of a purification process to remove the bulk of impurities.
• Description: Butyl-S Sepharose 6 Fast Flow belongs to the family of products referred to as hydrophobic interaction chromatography (HIC) media. Cytiva produces a wide range of HIC media that is well characterized for group separation or purification of a variety of biological macromolecules in laboratory- or process-scale operations. Butyl-S Sepharose 6 Fast Flow is the least hydrophobic medium in the series. Butyl-S Sepharose 6 Fast Flow is particularly useful during the initial stages of a separation process to remove the bulk of impurities without stringent requirements for conditioning of the sample It is a BioProcess medium that meets the demands of large-scale biopharmaceutical manufacturers for efficient and cost-effective protein purification.
• Characteristic: Designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations.
  Used in purification of recombinant Hepatitis B virus surface antigen from CHO cells.
  Minimal lot-to-lot variation • Hydrophilic backbone gives low non-specific interactions.
  Low risk of denaturation of relatively strong hydrophobic solutes.
• Maximum operating pressure: Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Sample preparation: The sample should be dissolved in start buffer. Alternatively the sample may be transferred to start buffer by dialysis or by buffer exchange using a HiTrap Desalting or a PD-10 Desalting columns. The viscosity of the sample should not exceed that of the buffer. For normal aqueous buffer systems, this corresponds to a protein concentration of approximately 50 mg/ml.
• Matrix: 6% cross-linked agarose
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 µm
• Ligand: Butyl-S
• Ligand density: Approx 10 µmol Butyl-S/ml medium
• Recommended flow rate: Approx. 400 cm/h at 1 bar (100 kPa, 14.5 psi), XK 50/30 column, bed height 15 cm.
• Recommended column height: 25 cm
• Chemical stability: Stable in commonly used aqueous buffers: 1 mM HCl, 1 M NaOH, 30% isopropanol, 50% ethylene glycol, 70% ethanol, 6 M guanidinehydrochloride, 8 M urea.
• pH working range: 3–13
• CIP stability: 2–14
• Temperature stability: 4°C to 40°C
• Autoclavable: 20 min at 121°C in 0.05 M sodium phosphate pH 7, 1 cycle.
• Storage: 4 to 30°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: Remove precipitated proteins: Wash the column with 4 bed volumes of 0.5–1.0 M NaOH solution at 40 cm/h, followed by 2–3 bed volumes of water.
  Remove tightly bound hydrophobic proteins, lipoproteins, and lipids: Wash the column with 4–10 bed volumes of up to 70% ethanol or 30% isopropanol. (Apply gradients to avoid the formation of air bubbles when using high concentrations of organic solvents.) Alternatively, wash the column with detergent in a basic or acidic solution, for example 0.5% non-ionic detergent in 1 M acetic acid. Wash at a flow rate of 40 cm/h. Residual detergent can be removed by washing with 5 bed volumes of 70% ethanol.
• Sanitization: Sanitization is the use of chemical agents to inactivate microbial contaminants. Sodium hydroxide (NaOH) is a commonly used sanitizing agent. A concentration of 0.5–1.0 M NaOH with a contact time of 30–60 min is effective for most microbial.
• Pack size: 200 mL
• BioProcess resin: Yes