Butyl Sepharose 4 Fast Flow

• Cat#No#: Bu-410C
• Product Overview: Butyl Sepharose 4 Fast Flow is a well established, standard aliphatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification of larger proteins.
• Description: Butyl Sepharose 4 Fast Flow is part of Cytiva's range of resins for hydrophobic interaction chromatography (HIC) and is designed for rapid processing of large volumes early in the downstream purification process. Butyl Sepharose 4 Fast Flow is a BioProcess resin and carries comprehensive technical and regulatory support for production scale applications.
• Characteristic: High dynamic capacity even at low salt concentrations.
  Excellent flow characteristics.
  No charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.
  Specially developed in co-operation with commercial pharmaceutical manufacturers.
  Easy scale-up.
• Maximum operating pressure: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20⁰C using buffers with the same viscosity as water)
• Sample preparation: The sample must be dissolved in start buffer. Alternatively the sample can be transferred to start buffer by dialysis or by buffer exchange using a HiTrap Desalting or a PD-10 Desalting column. The viscosity of the sample must not exceed that of the buffer. For normal aqueous buffer systems, this corresponds to a protein concentration of approximately 50 mg/mL.
  Before application the sample must be centrifuged or filtered through a 0.45 μm filter to remove any particulate matter.
• Packing Column: 1. Pour the resin slurry into the column in one continuous motion. Pouring down a glass rod held against the wall of the column helps prevent the introduction of air bubbles. Fill the remainder of the column with buffer.
  2. Wet the column adapter by submerging the plunger end in buffer, and drawing buffer through with a syringe or pump. Make sure that all bubbles have been removed. Disconnect the pump or syringe.
  3. Insert the adapter into the top of the column at an angle, taking care not to trap air under the net. Tighten the adapter O-ring to give a sliding seal on the column wall.
  4. Fit a syringe barrel to the sample application valve and connect the valve between the adapter and the pump.
  5. Open the bottom outlet of the column and start the pump.
  6. Stop the pump, close the column outlet, loosen the adapter O-ring to give a sliding seal and reposition the adapter on the surface of the resin bed. Press the adapter into the surface of the resin an additional 1 to 2 mm.
  7. Lock the adapter in position, open the column outlet and start the pump at the column packing flow rate.
• Matrix: cross-linked agarose, 4%, spherical
• Average particle size: ~90 μm
• Ligand: Butyl
• Ligand density: ~40 µmol Butyl/mL resin
• Recommended column height: 25 cm
• Chemical stability: Stable in commonly used aqueous buffers - 1.0 M NaOH, 30% isopropanol, 70% ethanol, 6 M guanidine-hydrochloride, 30% Acetonitrile, 1mM HCl.
• pH working range: 3–13
• CIP stability: 2–14
• Temperature stability: 4°C to 40°C
• Autoclavable: 20 min at 121 °C, 5 cycles in H₂O
• Storage: 4 to 30°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: Remove precipitated proteins: Wash the column with 4 bed volumes of 0.5–1.0 M NaOH solution at 40 cm/h, followed by 2–3 bed volumes of water.
  Remove tightly bound hydrophobic proteins, lipoproteins and lipids: Wash the column with 4–10 bed volumes of up to 70% ethanol or 30% isopropanol. Alternatively, wash the column with detergent in a basic or acidic solution. Wash at a flow velocity of 40 cm/h. Residual detergent can be removed by washing with 5 bed volumes of 70% ethanol.
• Sanitization: Sanitization is the use of chemical agents to inactivate microbial contaminants. Sodium hydroxide (NaOH) is a commonly used sanitizing agent. A concentration of 0.5–1.0 M NaOH with a contact time of 30–60 min is effective for most microbial contamination.
• Pack size: 200 mL
• BioProcess resin: Yes
• Dimensions: 5 cm