Butyl Sepharose High Performance

• Cat#No#: Bu-413C
• Product Overview: Butyl Sepharose High Performance is an aliphatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.
• Description: Butyl Sepharose High Performance is a member of the Cytiva range of HIC resins for intermediate purification and polishing of proteins. Butyl Sepharose High Performance is based on highly crosslinked, 34-µm agarose beads modified with aliphatic butyl groups via uncharged, chemically stable ether linkages. It is a truly hydrophobic resin displaying a minimum of ionic interactions. Butyl Sepharose High Performance resins is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.
• Characteristic: High-resolution, high-capacity separations with high recovery.
  Reliable and reproducible.
  High chemical stability for effective CIP and sanitization.
  Available in laboratory and BioProcess scale quantities.
  Easy to scale up.
• Maximum operating pressure: Base matrix: 100-200 cm/h, 300 kPa, BioPilot 60/600 column, bed height 30 cm.
• Matrix: cross-linked agarose
• Particle Size: 24 µm-44 µm
• Average particle size: ~34 µm
• Ligand: Butyl
• Ligand density: Approx. 50 µmol butyl/ml gel
• Dynamic binding capacity: ~ 38 mg β-lactoglobulin/mL resin
• Recommended flow rate: ≤ 100 cm/h
• Recommended column height: 30 cm
• Chemical stability: Stable in commonly used aqueous buffers - 1 M sodium hydroxide, 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% acetonitrile, 70% ethanol, 3 M ammoniumsulfate, 1 mM HCl, 30% isopropanol, 2% SDS
• pH working range: 3–13
• CIP stability: 2–14
• Cleaning-in-place: Removal of precipitated proteins: Wash the column with 4 bed volumes of 0.01 M NaOH at 40 cm/h, followed by 2 to 3 bed volumes of water.
  Removal of tightly bound hydrophobic proteins, lipoproteins and lipids: Wash the column with 4 to 10 bed volumes of up to 70% ethanol or 30% isopropanol followed by 3 to 4 bed volumes of water.
  Alternatively, wash the column with detergent in a basic or acidic solution, for example, 0.5% nonionic detergent in 1 M acetic acid. Wash at a flow velocity of 40 cm/h. Remove residual detergent with 5 bed volumes of 70% ethanol followed by 3 to 4 bed volumes of water.
• Sanitization: Wash the column with 0.01 M NaOH at a flow velocity of approximately 40 cm/h, contact time 30 to 60 min.
• Pack size: 25 mL
• BioProcess resin: Yes
• Maximum flow velocity: 4.0 mL/min (1 mL), 20 mL/min (5 mL)
• Dimensions: 0.7 × 2.5 cm (1 mL), 1.6 × 2.5 cm (5 mL)
• Column volume: 1 mL and 5 mL
• Column hardware pressure limit: 5 bar (0.5 MPa, 73 psi)