Calmodulin Sepharose 4B

• Cat#No#: Ca-350C
• Product Overview: Calmodulin Sepharose 4B affinity resin for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells.
  For single-step purification of native calmodulin-binding proteins.
  Suitable for tandem affinity purification (TAP) of protein complexes.
  Purification of calmodulin-regulated proteins from all eukaryotic cells.
• Description: Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD₊/NADP₊ Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca₂₊ on separate Ca₂₊-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca₂₊ complexes are particularly stable.
• Medium Preparation: Calmodulin Sepharose 4B is supplied pre-swollen in 20% ethanol. Prepare a slurry by decanting the 20% ethanol solution and replace it with binding buffer in a ratio of 75% settled medium to 25% buffer. The binding buffer should not contain agents which significantly increase the viscosity. The column may be equilibrated with viscous buffers at reduced flow rates after packing is completed.
• Ligand Coupling Method: Cyanogen bromide activation
• Matrix: Agarose, 4%
• Particle Size: 45 μm-165 μm
• Average particle size: ~90 µm
• Ligand: Calmodulin
• Recommended flow rate: < 75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height
• Chemical stability: Stable to commonly used aqueous solutions
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• pH working range: 4–9
• CIP stability: 4–9
• Storage: 2 to 8°C, 20% Ethano
• Cleaning-in-place: In some applications, substances such as denaturated proteins or lipids do not elute in the regeneration procedure. These can be removed by washing the medium with a nonionic detergent, at 37°C for one minute followed by reequilibration with 3 column volumes of binding buffer.
• Pack size: 10 mL