Cat# | Product Name | Size | Price | Qty | Inquiry |
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Ca-469C | Capto adhere ImpRes resin | Inquiry |
Product Information | |
Cat#No# | Ca-469C |
Product Overview | This multimodal, strong anion exchange resin supports effective mAb polishing in the second or third step of a purification scheme downstream of the protein A capture step. |
Description | Capto adhere ImpRes is a BioProcess chromatography medium (resin) for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. The strong anion exchange multimodal ligand displays high selectivity compared with traditional ion exchangers, which allows the possibility to solve challenging purification problems. Main contaminants in MAb processes such as DNA, host cell proteins (HCP), leached protein A, aggregates, and viruses are efficiently separated. Capto adhere ImpRes is an excellent choice for purification of MAb with high aggregate content. The efficient virus removal provided by the chromatography medium also allows two-step purification of MAbs with Capto adhere ImpRes as the final polishing step. Polishing can be performed in either bind and elute (binding) or flowthrough (nonbinding) modes. |
Characteristic | High yields achieved through the high-resolution beads and selectivity of the ligand. Efficient removal of aggregates, viruses, and main contaminants in mAb processes. Enables use of a platform approach to mAb process development. Allows separation of mAb charged variants. Resin fulfills industrial demands for security of supply, robust performance, and regulatory support |
Maximum operating pressure | 300 kPa |
Metal ion capacity | 0.08-0.11 mmol Cl-/ml medium |
Matrix | High-flow agarose |
Particle Size | 36–44 μm |
Average particle size | ~40 μm |
Ligand | Multimodal strong anion exchanger |
Dynamic binding capacity | 45-85 mg Mab/mL |
Recommended flow rate | 100 to 300 cm/h (HiScreen) and 25 to 50 cm/h (HiTrap) |
Recommended column height | 20 cm |
Chemical stability | Commonly used aqueous buffers, 1 M acetic acid, 1 M NaOH, 2 M NaCl, 5% 1-propanol, 30% isopropanol, 70% ethanol. |
pH working range | 3–12 |
CIP stability | 2–14 |
Temperature stability | 4°C to 30°C |
Storage | 4°C to 30°C in 20% ethanol |
Cleaning-in-place | Precipitated hydrophobically bound proteins or lipoproteins: Wash with at least 3 column volumes of 1 M NaOH at a low flow velocity with reversed flow direction. Contact time at least 15 min. Ionically bound proteins: Wash with 0.5 to 2 column volumes of 2 M NaCl with reversed flow direction. Lipids and very hydrophobic proteins: Wash with 1-propanol 1 to 5% or isopropanol 5 to 30%. 1-propanol has a higher flash point and might be preferred in an industrial environment. Nucleic acids: Wash with 0.1 M acetate, pH 3 for 2 to 5 column volumes followed by buffer at neutral pH for 1 to 2 column volumes. Then wash with at least 3 column volumes 1 M NaOH at a low flow velocity with reversed flow direction. Contact time 15 min. |
Sanitization | To reduce microbial contamination in the packed column, sanitization using 0.5 to 1 M NaOH with a contact time of 1 hour is recommended. |
Pack size | 25 mL |
BioProcess resin | Yes |
Maximum flow velocity | 300 cm/h |
Dimensions | 1 m |
Functional group | multimodal strong anion exchanger |
Download Datasheet | Download SDS |
For Research or Industrial Raw Materials, Not For Personal Medical Use!
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