Capto adhere in ReadyToProcess colum

• Cat#No#: Ca-282P
• Product Overview: These ReadyToProcess chromatography columns are prepacked with Capto adhere chromatography resin and are excellent for intermediate purification polishing and purification after protein A capture. ReadyToProcess columns are validated high-performance bioprocessing columns that are supplied prepacked and ready for use.
• Characteristic: Wide operational window of pH and conductivity.
  High capacity and productivity in flow-through mode.
  Two-step chromatographic process with protein A affinity, saves time and cuts operating costs.
  No cross contamination.
  Removes contaminants to formulation levels.
• Applications: For manufacturing of biopharmaceuticals for clinical phase I and II studies. Depending on the scale of operations, they can also be used for full-scale manufacturing, as well as for preclinical studies.
• Maximum operating pressure: 3 bar [0.3 MPa] (44 psi)
• Metal ion capacity: 0.09 to 0.12 mmol Cl- /mL resin
• Matrix: Highly cross-linked agarose, spherical
• Average particle size: ~ 75 μm
• Recommended column height: 200 mm (7.87 in)
• Chemical stability: Stable to commonly used aqueous buffers, 1 M acetic acid, 1.0 M NaOH.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Autoclavable: 17 min at 121°C in 0.05 M phosphate buffer, pH 7, 10 cycles.
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Precipitated hydrophobically bound proteins or lipoproteins: Wash with 1.0 M NaOH at 150 cm/h with reversed flow direction. Contact time 15 to 30 minutes.
  Ionically bound proteins: Wash with 0.5 to 2 column volumes of 2 M NaCl with reversed flow direction.
  Lipids and very hydrophobic proteins: Wash with 1-propanol 1 to 5% or isopropanol 5 to 30%. 1-propanol has a higher flash point and might be preferred in an industrial environment.
  Nucleic acids: Wash with 0.1 M acetate pH 3 for 2 to 5 column volumes followed by equilibration buffer at neutral pH for 1 to 2 column volumes and wash 1.0 M NaOH at 150 cm/h with reversed flow direction. Contact time 15 to 30 minutes.
• Sanitization: To reduce microbial contamination in the packed column, sanitization using 0.5 to 1.0 M NaOH with a contact time of 1 hour is recommended. The CIP protocol for precipitated, hydrophobic bound proteins or lipoproteins removes bound contaminants and sanitizes the resin effectively.
• Pack size: 2.5 L
• Dimensions: 126 × 200 mm
• Column volume: 2.5 L
• Column i.d.: 126 mm
• Functional group: Multimodal strong anion exchanger