Capto adhere resin

• Cat#No#: Ca-470C
• Product Overview: A multimodal anion exchange resin designed for post-protein A purification of monoclonal antibodies at process scale. It can also be used in bind/elute mode.
• Description: Capto adhere is a multimodal BioProcess resin designed for post-protein A purification of monoclonal antibodies (mAbs) at process scale. The strong ion exchange multimodal ligand gives a different selectivity compared with traditional ion exchangers. Capto adhere can remove key impurities such as DNA, host cell proteins (HCP), leached protein A, aggregates, and viruses in a single step, allowing the design of a two-step process together with MabSelect SuRe protein A resin. If necessary, Capto adhere can be used in combination with anion or cation exchange chromatography for polishing, as a second or third step in any mAb purification platform.
• Characteristic: Wide operational window of pH and conductivity.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
  Two-step chromatographic process with protein A affinity, saves time and cuts operating costs.
  Removes contaminants to formulation levels.
  High capacity and productivity in flow-through mode.
• Maximum operating pressure: 300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height
• Metal ion capacity: 0.09-0.12 mmol Cl-/ml medium
• Matrix: Highly cross-linked agarose
• Ionic Exchanger Type: Multimodal strong anion exchanger
• Average particle size: ~75 µm
• Ligand: N-benzyl-n-methyl ethanolamine
• Recommended column height: 20 cm
• Chemical stability: Commonly used aqueous buffers, 1 M acetic acid, 1 M sodium hydroxide.
• pH working range: 3–12
• CIP stability: 2–14
• Temperature stability: 4°C to 30°C
• Autoclavable: 17 min at 121°C in 0.05 M phosphate buffer, pH 7, 10 cycles.
• Shelf life: 5 years
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Precipitated hydrophobically bound proteins or lipoproteins: Wash with 1.0 M NaOH at 150 cm/h with reversed flow direction. Contact time 15 to 30 minutes.
  Ionically bound proteins: Wash with 0.5 to 2 column volumes of 2 M NaCl with reversed flow direction.
  Lipids and very hydrophobic proteins:Wash with 1-propanol 1 to 5% or isopropanol 5 to 30%. 1-propanol has a higher flash point and might be preferred in an industrial environment.
  Nucleic acids: Wash with 0.1 M acetate pH 3 for 2 to 5 column volumes followed by equilibration buffer at neutral pH for 1 to 2 column volumes and wash 1.0 M NaOH at 150 cm/h with reversed flow direction. Contact time 15 to 30 minutes.
• Sanitization: To reduce microbial contamination in the packed column, sanitization using 0.5 to 1.0 M NaOH with a contact time of 1 hour is recommended.
• Pack size: 100 mL
• BioProcess resin: Yes
• Dimensions: 1 m
• Functional group: Multimodal strong anion exchanger