Capto Blue affinity chromatography resin

• Cat#No#: Ca-353C
• Product Overview: Capto Blue affinity resin with Cibacron Blue ligand has outstanding pressure and flow properties, allowing rapid processing of large sample volumes of albumin and other proteins.
  Excellent chemical stability ensures tolerance of harsh solvents used in repeated cleaning-in-place and sanitization procedures.
  Capto Blue can be autoclaved repeatedly.
  Highly rigid agarose base matrix allows high flow rates and processing of large sample volumes with no reduction in binding capacity. Ligand functionality can be modified through the use of appropriate buffer salts and buffer conductivity to increase selectivity for desired targets.
  Excellent choice for the removal or purification of proteins at both laboratory and process scales.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Description: Capto Blue is affinity chromatography media (resins) for the capture of human serum albumin (HSA), as well as purification of HSA fusion proteins, blood coagulation factors, enzymes, and recombinant proteins in laboratory and process scales. Developed from Blue Sepharose 6 Fast Flow, Capto Blue products are more chemically stable and have a more rigid agarose base matrix than their predecessor. These improvements allow the use of higher flow rates and larger sample volumes, enabling increased throughput and improved process economy.
• Characteristic: Excellent chemical stability for tolerance to the harsh solvents used in repeated cleaning-in-place (CIP) and sanitization procedures. Highly rigid agarose base matrix allows high flow rates and processing of large sample volumes.
  Ligand functionality may be modified through the choice of buffer salt and conductivity to increase selectivity for desired targets.
  Excellent choice for the removal or purification of proteins in both laboratory and process scales.
• Applications: Capture of human serum albumin (HSA).
  Purification of HSA fusion proteins.
  Purification of blood coagulation factors, enzymes, and recombinant proteins.
• Maximum operating pressure: 300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height
• Sample preparation: HSA, 1 mg/mL
• Medium Preparation: Capto Blue media are based on a highly rigid agarose base matrix that offers outstanding pressure and flow properties, allowing rapid processing of large sample volumes. The Cibacron Blue ligand is attached to the base matrix via a hydrophilic spacer and is immobilized with a stable amine bond.
• Ligand Coupling Method: Ether linkages and hydrophilic spacer arm
• Packing Column: All large-scale columns can be supplied as variable bed height columns. Do not choose large diameter columns if the bed height is low.
• Column: Tricorn 5/100 (10 cm bed height)
• Matrix: Highly cross-linked agarose
• Average particle size: ~75 µm
• Ligand: Cibacron blue
• Ligand density: Approx. 13 μmol/ml
• Dynamic binding capacity: > 24 mg HSA/mL resin (Qb10 at 4 min residence time)
• Recommended flow rate: 300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height
• Recommended column height: 20 cm
• Chemical stability: Stable to commonly used aqueous buffers:0,5 M NaOH, 8 M urea, 6 M guanidine hydrochloride.
• pH working range: 2–13
• CIP stability: 2–13
• Storage: 2 to 8°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol
• Cleaning-in-place: Remove precipitated proteins by:1.washing the column with 4 column volumes (CV) of 0.5 M NaOH at 40 cm/h, followed by washing the column with 3–4 CV of 70% ethanol or 2 M potassium thiocyanate. 2.Alternatively, wash the column with 2 CV of 6 M guanidine hydrochloride. 3.In both cases wash immediately with at least 5 CV filtered binding buffer, pH 8.0.
  Remove strongly bound hydrophobic proteins, lipoproteins and lipids by:1.washing the column with 3–4 CV of up to 70% ethanol or 30% isopropanol. 2.Alternatively, wash the column with 2 CV detergent in a basic or acidic solution, e.g., 0.1% non-ionic detergent in 1 M acetic acid. Wash at a flow rate of 40 cm/h. Remove residual detergent by washing with 5 CV of 70% ethanol. 3.In both cases wash immediately with at least 5 CV filtered binding buffer, pH 8.0.
• Sanitization: Capto Blue (high sub) can also be sanitized by autoclaving, which is particularly appropriate if microbial contamination is suspected. When Capto Blue (high sub) was autoclaved 10 times (121°C, 2.5 to 2.7 bar), no decrease in ligand density was observed. Capto Blue has not been exposed to autoclaving but is expected to exhibit the same tolerance as Capto Blue (high sub).
• Pack size: 25 mL
• BioProcess resin: Yes
• Dimensions: 1 m