Capto Butyl ImpRes resin

• Cat#No#: Ca-415C
• Product Overview: Capto Butyl ImpRes is a HIC resin for the intermediate and polishing steps in a downstream purification process.
• Description: Capto Butyl ImpRes is hydrophobic interaction chromatography (HIC) media (resins) developed for the intermediate and polishing steps in a downstream protein purification process. Both chromatography media extend the well established Capto platform to include high-resolution media. By combining the high-flow characteristics of Capto media with a smaller particle size, Capto Butyl ImpRes deliver both excellent pressure/flow properties and resolution. The ability to run at higher flow velocities and higher bed heights increases flexibility in process design and might enable increased productivity.
• Characteristic: High-resolution purification based on Capto ImpRes base matrix with a traditional HIC ligand and smaller average particle size. Flexible process design due to a large operational window of flow velocities and bed heights.
  Higher throughput compared with Capto Butyl might enable improved productivity and process economy.
  Excellent chemical stability.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Maximum operating pressure: Up to 220 cm/h in a 1 m diameter column with a bed height of 20 cm at 20°C using process buffers with the same viscosity as water at 300 kPa.
• Matrix: High Flow Agarose
• Average particle size: ~40 µm
• Ligand: Butyl
• Dynamic binding capacity: 37 mg BSA /mL
• Recommended flow rate: Up to 220 cm/h in a 1 m diameter column with a bed height of 20 cm at 20°C; measured using process buffers with the same viscosity as water at 300 kPa.
• Recommended column height: 20 cm
• Chemical stability: Stable in commonly used aqueous buffers, 1 M sodium hydroxide.
• Shelf life: 5 years
• Storage: 20% ethanol at 4°C to 30°C
• Cleaning-in-place: General recommendation for CIP and sanitization protocols for all Cytiva HIC media is to use 1 M NaOH. Use of a water-diluted organic solvent, such as ethanol or isopropanol, can be efficient in breaking strong hydrophobic interactions during CIP.
• Sanitization: General recommendation for CIP and sanitization protocols for all Cytiva HIC media is to use 1 M NaOH. Use of a water-diluted organic solvent, such as ethanol or isopropanol, can be efficient in breaking strong hydrophobic interactions during CIP.
• Pack size: 25 mL
• BioProcess resin: Yes
• Dimensions: 1 m