Capto Chelating

• Cat#No#: Ca-354C
• Product Overview: Capto Chelating is a BioProcess immobilized metal ion affinity chromatography (IMAC) resin.
  Rigid agarose base matrix allows high flow rates and processing of large sample volumes for increased throughput.
  Capto Chelating is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.
  Convenient purification of native and recombinant proteins with affinity for metal ions.
• Description: Capto Chelating chromatography medium (resin) is a BioProcess medium for immobilized metal ion affinity chromatography (IMAC) of native and histidine-tagged fusion proteins. The medium is suitable for both laboratory- and large-scale purifications. Capto Chelating is part of a platform of media based on the Capto product line.
• Characteristic: Suitable for a wide range of purification applications.
  Straightforward scale-up to production columns.
  Withstands effective and rigorous cleaning-in-place (CIP) procedures.
• Ligand Coupling Method: Allylation-Bromination
• Matrix: Highly cross-linked agarose
• Average particle size: ~75 µm
• Ligand: Iminodiacetic acid
• Ligand density: 23-33 µmol/ml
• Recommended flow rate: Minimum 600 cm/h in a 1 m diameter column with 20 cm bed height at 20°C using process buffers with the same viscosity as water at < 3 bar (0.3 MPa).
• Chemical stability: pH 2-14
• pH working range: 3–12
• CIP stability: 2–14
• Shelf life: 5 years
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: 1.Removing ionically bound proteins by washing the column with 0.5 column volumes of a 2 M NaCl solution.
  2.Removing precipitated proteins, hydrophobically bound proteins, and lipoproteins by washing the column with 1 M NaOH.
  3.Removing strongly hydrophobically bound proteins, lipoproteins, and lipids by washing the column with 70% ethanol, 30% isopropanol, or detergents in a basic or acidic solution.
• Sanitization: Sanitization reduces microbial contamination of the medium. A recommended sanitization procedure is treatment with 0.5–1 M NaOH.
• Pack size: 25 mL
• BioProcess resin: Yes