Capto Core 400

• Cat#No#: Ca-465C
• Product Overview: Novel core bead technology and multimodal ligand give Capto Core 400 dual functionality, size exclusion and binding chromatography, in one chromatography resin.
• Description: Capto Core 400 multimodal chromatography resins are designed for intermediate purification and polishing of viruses and other large biomolecules. The core bead technology and multimodal, octylamine ligand give the resins dual‑functionality: size separation and binding chromatography in one chromatography resin. These features make Capto Core 400 excellent alternatives to size‑exclusion chromatography (SEC) resins that are typically employed in the final stages of virus purification in vaccine manufacture. Capto Core 400 offer a range of performance advantages over SEC, which is often regarded as a productivity bottleneck in the polishing process due to low flow rates and limited sample loads.
• Characteristic: Significantly improved productivity enabled by up to 100‑fold higher sample load and significantly higher flow rates compared with SEC. Bead with ligand‑activated core and inactive shell allows efficient capture of impurities, while target molecules are collected in the flowthrough.
  Straightforward optimization due to flow-through chromatography and robust performance, allowing for a wide window of operation.
• Metal ion capacity: 40-85 µmol/mL(Cl-)
• Matrix: Highly cross‑linked agarose
• Average particle size: 90 μm
• Ligand: Octylamine
• Dynamic binding capacity: 22 mg ovalbumin/mL resin
• Recommended flow rate: 700 cm/h
• Recommended column height: 20 cm
• Chemical stability: All commonly used aqueous buffers, 1 M sodium hydroxide (NaOH)§, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
• pH working range: 3 to 13
• CIP stability: 3 to 14
• Cleaning-in-place: 1 M NaOH in 27% 1‑propanol
• Sanitization: 1 M NaOH in 27% 1‑propanol
• Pack size: 25 mL
• BioProcess resin: Yes
• Maximum flow velocity: 700 cm/h in column with 20 cm bed height at < 2 bar (0.2 MPa)