Capto Core 700 resin

• Cat#No#: Ca-466C
• Product Overview: Novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality. This chromatography resin has both size exclusion and binding properties.
• Characteristic: Novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality. This chromatography resin has both size exclusion and binding properties.
  Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough. Significantly improved productivity and higher flow rates compared with standard size exclusion (gel filtration) methods. Straightforward optimization due to flowthrough chromatography and robust performance.
  Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Metal ion capacity: 40-85 µmol/ml (Cl-)
• Matrix: Highly cross-linked agarose
• Average particle size: ~90 µm
• Ligand: Octylamine
• Dynamic binding capacity: 13 mg ovalbumin
• Recommended flow rate: < 500 cm/h
• Chemical stability: Commonly used aqueous buffers,1 M sodium hydroxide6, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
• pH working range: 3–13
• CIP stability: 2–14
• Temperature stability: 4ºC to 30ºC
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Wash with a solution of 1 M NaOH in 30% isopropanol or in 27% 1-propanol with reversed flow direction. Use a total contact time of 30 to 60 min.
• Sanitization: To reduce microbial contamination in the packed column, sanitization using 0.5 to 1 M NaOH with a contact time of 1 h is recommended. The CIP protocol described above with 1 h contact time sanitizes the medium effectively.
• Pack size: 25 mL
• BioProcess resin: Yes
• Maximum flow velocity: 500 cm/h in columns with 20 cm bed height at <2 bar (0.2 MPa)
• Functional group: CH3CH2CH2CH2CH2CH2CH2CH2NH