Capto DEAE resin

• Cat#No#: Ca-441C
• Product Overview: Capto DEAE is a weak anion exchanger for large-scale manufacturers. Meets demands for fast, cost-effective capture and intermediate protein purification.
• Description: Capto DEAE is strong cation, strong anion, and weak anion exchange resins for packed bed chromatography that increase speed and throughput in capture and intermediate purification. They combine high capacity with high flow velocity and low back pressure to reduce process cycle times and increase productivity.
• Characteristic: Weak anion exchanger for high-productivity capture and intermediate purification when high volume throughput is essential.
  High dynamic binding capacity at high flow raises productivity.
  High-volume throughput cuts process times.
  Cost-effective processing with smaller unit operations.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Maximum operating pressure: 300 kPa at 700 cm/h, 1 m diameter column.
• Ligand Coupling Method: Dextran surface extenders.
• Metal ion capacity: 0.29-0.35 mmol Cl-/ml medium
• Matrix: Highly cross-linked agarose with dextran surface extender.
• Ionic Exchanger Type: Weak anion exchanger
• Average particle size: ~90 μm
• Ligand: Diethylaminoethyl
• Dynamic binding capacity: > 90 mg ovalbumin/mL resin
• Recommended flow rate: 700 cm/h
• Recommended column height: 20 cm
• Chemical stability: Commonly used aqueous buffers, 1 M acetic acid, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol
• pH working range: 2–9
• CIP stability: 2–14
• Temperature stability: 4°C to 30°C
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Precipitated, hydrophobically bound proteins or lipoproteins: Wash with 1.0 M NaOH at 40 cm/h with reversed flow direction. Contact time 1 to 4 h, dependent on feed. If the removal of contaminants is not satisfactory for Capto DEAE, use 1.0 M NaOH containing 1 M NaCl as CIP solution.
  Ionically bound proteins: Wash with 0.5 to 2 column volumes (CV) of 2 M NaCl with reversed flow direction. Contact time 10 to 15 min.
  Lipids and very hydrophobic proteins: Wash with 2 to 4 CV of up to 70% ethanol1 or 30% isopropanol with reversed flow direction. Contact time 1 to 2 h, dependent on feed. Alternatively, wash with 2 to 4 CV of 0.1% nonionic detergent with reversed flow direction. Contact time 1 to 2 h, dependent on feed.
• Sanitization: To reduce microbial contamination in the packed column, sanitization using 0.5 to 1.0 M NaOH with a contact time of 1 h is recommended. The CIP protocol for precipitated, hydrophobically bound proteins or lipoproteins sanitizes the resin effectively.
• Pack size: 100 mL
• BioProcess resin: Yes