Capto Heparin affinity chromatography resin

• Cat#No#: Ca-356C
• Product Overview: Capto Heparin is a resin for packed-bed affinity chromatography. It is used to purify coagulation factors, plasma proteins, DNA binding proteins, lipoproteins, and enzymes:
  Outstanding pressure and flow rate properties.
  Excellent selectivity.
  High chemical stability.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Description: Capto Heparin, a medium for packed-bed affinity chromatography, gives fast and reliable separations of biomolecules with an affinity for heparin. These include coagulation factors, antithrombin III, and other plasma proteins, DNA binding proteins, lipoproteins, protein synthesis factors, and enzymes. Capto Heparin media is available as bulk media and in prepacked HiPrep columns.
• Characteristic: Outstanding pressure and flow rate properties.
  Excellent selectivity.
  High chemical stability.
• Applications: For using Capto Heparin to purify the blood coagulation factor antithrombin III from bovine plasma.
• Maximum operating pressure: 300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height
• Ligand Coupling Method: Reductive amination
• Matrix: Highly cross-linked agarose
• Average particle size: ~90 μm
• Ligand: Heparin
• Ligand density: Approx. 1.8 mg heparin/ml medium
• Dynamic binding capacity: Approx. 1.4 mg ATIII/mL resin
• Recommended flow rate: 300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height
• Recommended column height: 20 cm
• Chemical stability: Stable to commonly used aqueous buffers.
• pH working range: 4–12
• CIP stability: 4–13
• Storage: 4 to 30°C, in 20% Ethanol and 0.05 M Sodium Acetate
• Cleaning-in-place: Substances such as denatured proteins that do not elute during regeneration can be removed by cleaning-in-place (CIP) procedures. A recommended CIP procedure for Capto Heparin is 4 CV of 0.1 M NaOH with a contact time of 1 to 2 h.
• Sanitization: Sanitization reduces microbial contamination of the medium. A recommended sanitization procedure for Capto Heparin is to treat the packed column with 0.1 M NaOH and 20% ethanol for 1 h, or to allow it to stand in 70% ethanol for 12 h. Always wash the packed column with equilibration buffer after sanitization.
• Pack size: 25 mL
• BioProcess resin: No
• Wash buffer: 0.1 M Tris-HCl, 0.01 M trisodium citrate, 0.330 M NaCl, pH 7.4
• Dimensions: 1 m