Capto Lentil Lectin affinity chromatography resin

• Cat#No#: Ca-357C
• Product Overview: Capto Lentil Lectin resin is designed for purification of glycoproteins and other molecules containing carbohydrates, such as α-D-mannose and α-D-glucose or related residues:
  Group-specific adsorbent for carbohydrate-containing molecules.
  High productivity and cost efficiency in downstream operations.
  Animal component-free production.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Description: Capto Lentil Lectin is an affinity chromatography resin for purification of glycoproteins and other molecules containing carbohydrates such as α-D-mannose and α-D-glucose or sterically related residues. The product is based on a rigid high-flow agarose base matrix that enables high flow rates. Capto Lentil Lectin resin is available in bulk as well as in various formats for high-throughput process development.
• Characteristic: Group-specific adsorbent for carbohydrate-containing molecules.
  High productivity and cost-efficiency in downstream operations.
  Animal component-free production.
  Security of supply and regulatory support.
• Maximum operating pressure: < 300 cm/h, < 200 kPa, BPG 300, bed height 20 cm
• Matrix: Highly cross-linked agarose
• Average particle size: ~75 µm
• Ligand: Lentil Lectin
• Ligand density: 3 mg/ml medium
• Coupling chemistry: NHS
• Dynamic binding capacity: ~ 15 mg porcine thyroglobulin/mL resin
• Recommended flow rate: 100 to 300 cm/h
• Recommended column height: 20 cm
• Chemical stability: Stable to commonly used aqueous buffers. Chelating agents such as EDTA, 8 M urea or solutions having a pH below 3 should be avoided as these conditions result in removal of manganese from the lectin and loss of activity.
• pH working range: 3–10
• CIP stability: 3–10
• Shelf life: 3 years
• Storage: 2 to 8°C in 20% ethanol, 150 mM NaCl, 1 mM Ca²⁺ and 1 mM Mn²⁺
• Pack size: 25 mL
• Maximum flow velocity: 300 cm/h