Capto MMC ImpRes resin

• Cat#No#: Ca-467C
• Product Overview: Designed for modern, large-scale manufacturers, this resin is used for high-resolution polishing of monoclonal antibodies (mAbs) and other biomolecules.
• Description: Capto MMC ImpRes is a BioProcess chromatography medium (resin) for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. The weak cation exchange multimodal ligand enables high binding selectivity in a broad pH/salt window compared with traditional ion exchangers, which allows the possibility to solve challenging purification problems. Capto MMC ImpRes is an excellent choice for removal of aggregates and host cell proteins (HCP) in MAb processes. The wide window of operation for conductivity and/or pH simplifies the process as conditioning after the protein A step can be omitted. When working with sensitive MAbs, the wide window of operation also simplifies optimization of conditions. Capto MMC ImpRes can be used in the initial polishing step in MAb purification processes, as well as for polishing of antibody fragments such as domain antibodies (DAbs).
• Characteristic: High yields achieved through the high-resolution beads and selectivity of the ligand.
  Efficient removal of aggregates, viruses, and main contaminants in mAb processes.
  Enables use of a platform approach to mAb process development.
  Displays a broad operational window for pH and salt.
  Suitable for polishing of antibody fragments.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Maximum operating pressure: 300 kPa
• Metal ion capacity: 25 to 39 µmol/mL
• Particle Size: 36 to 44 μm
• Average particle size: ~40 μm
• Ligand: Multimodal weak cation exchanger
• Ligand density: 25-39 µmol/ml
• Dynamic binding capacity: 60-90 mg Mab/mL
• Recommended flow rate: 220 cm/h
• Recommended column height: 20 cm
• Chemical stability: Commonly used aqueous buffers, 1 M acetic acid, 1 M NaOH, 2 M NaCl, 5% 1-propanol, 30% isopropanol, 70% ethanol.
• pH working range: 3–12
• CIP stability: 2–14
• Temperature stability: 4°C to 30°C
• Storage: 4°C to 30°C in 20% ethanol, 0.2 M sodium acetate.
• Cleaning-in-place: Precipitated hydrophobically bound proteins or lipoproteins: Wash with at least 3 column volumes of 1 M NaOH at a low flow velocity with reversed flow direction. Contact time at least 15 min.
  Ionically bound proteins: Wash with 0.5 to 2 column volumes of 2 M NaCl with reversed flow direction.
  Lipids and very hydrophobic proteins: Wash with 1-propanol 1 to 5% or isopropanol 5 to 30%. 1-propanol has a higher flash point and might be preferred in an industrial environment.
  Nucleic acids: Wash with 0.1 M acetate, pH 3 for 2 to 5 column volumes followed by buffer at neutral pH for 1 to 2 column volumes. Then wash with at least 3 column volumes 1 M NaOH at a low flow velocity with reversed flow direction. Contact time 15 min.
• Sanitization: To reduce microbial contamination in the packed column, sanitization using 0.5 to 1 M NaOH with a contact time of 1 hour is recommended.
• Pack size: 25 mL
• BioProcess resin: Yes
• Maximum flow velocity: at least 220 cm/h in a 1 m diameter column with 20 cm bed height at 20°C; using process buffers with the same viscosity as water at < 3 bar (0.3 MPa)
• Dimensions: 1 m
• Functional group: multimodal weak cation exchanger