Capto MMC resin

• Cat#No#: Ca-468C
• Product Overview: Capto MMC is a multimodal weak cation exchanger that is salt-tolerant. It has a different selectivity, compared with traditional ion exchangers.
• Description: Capto MMC is a multimodal cation exhanger in the Capto family of BioProcess resins for fast, efficient and cost-effective protein purification. Capto MMC combines agarose base matrix developments with innovative ligand chemistry. The adsorption is salt tolerant, meaning that binding of proteins can be performed at the conductivity of the feed material. The resin is based on a highly rigid agarose base matrix that allows high flow rates and low back pressure at large scale.
• Characteristic: Multimodal cation exchanger with a different selectivity than traditional ion exchangers.
  High dynamic binding capacity at high conductivity increases productivity.
  High-volume throughput cuts process times.
  Smaller unit operations give cost-effective processing and reduce investments.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Maximum operating pressure: 300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height
• Metal ion capacity: 0.07-0.09 mmol H+/ml medium
• Matrix: Highly cross-linked agarose
• Ionic Exchanger Type: Multimodal weak cation exchanger
• Average particle size: ~75 µm
• Dynamic binding capacity: > 45 mg BSA/mL resin at 30 mS/cm
• Recommended column height: 20 cm
• Chemical stability: Commonly used aqueous buffers, 1 M acetic acid, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, and 70% ethanol.
• pH working range: 2–12
• CIP stability: 2–14
• Temperature stability: 4°C to 30°C
• Autoclavable: 17 min at 121°C in 0.05 M phosphate buffer, pH 7, 10 cycles.
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Precipitated, hydrophobically bound proteins or lipoproteins: Wash with 1.0 M NaOH at 40 cm/h with reversed flow direction. Contact time 1 to 2 hours, dependent on feed.
  Ionically bound proteins: Wash with 0.5 to 2 column volumes of 2 M NaCl with reversed flow direction. Contact time 10 to 15 min. Lipids and very hydrophobic proteins: Wash with 2 to 4 column volumes of up to 70% ethanol1, or 1-propanol 1% to 5%, or 30% iso-propanol with reversed flow direction. Contact time 1 to 2 hours, dependent on feed. 1-propanol has higher flash point and might be preferred in an industrial environment. Alternatively, wash with 2 to 4 column volumes of 0.1% nonionic detergent with reversed flow direction. Contact time 1 to 2 hours, dependent on feed.
• Sanitization: To reduce microbial contamination in the packed column, sanitization using 0.5 to 1.0 M NaOH with a contact time of 1 hour is recommended.
• Pack size: 25 mL
• BioProcess resin: Yes
• Dimensions: 1 m
• Functional group: Multimodal weak cation exchanger