Capto Phenyl ImpRes resin

• Cat#No#: Ca-419C
• Product Overview: Capto Phenyl ImpRes is a HIC resin for high-resolution intermediate and polishing steps in a downstream purification process.
  Combines the high-flow characteristics of Capto resins with a smaller particle size to deliver both excellent pressure/flow properties and resolution.
  Flexible process design due to a large operational window of flow velocities and bed heights.
  Higher throughput might enable improved productivity and process economy.
  Excellent chemical stability allows cleaning with standard CIP solutions, including 1 M sodium hydroxide.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Description: Capto Phenyl ImpRes is hydrophobic interaction chromatography (HIC) media (resins) developed for the intermediate and polishing steps in a downstream protein purification process. Both chromatography media extend the well established Capto platform to include high-resolution media. By combining the high-flow characteristics of Capto media with a smaller particle size, Capto Phenyl ImpRes and Capto Butyl ImpRes deliver both excellent pressure/flow properties and resolution. The ability to run at higher flow velocities and higher bed heights increases flexibility in process design and might enable increased productivity.
• Characteristic: High-resolution intermediate and polishing purification based on Capto ImpRes base matrix with traditional HIC ligands.
  Flexible process design due to a large operational window of flow velocities and bed heights.
  Higher throughput may enable improved productivity and process economy.
  Excellent chemical stability.
  Security of supply and comprehensive regulatory support.
• Maximum operating pressure: 300 kPa at min. 220 cm/h, 1 m diameter column, 20 cm bed height.
• Matrix: High Flow Agarose
• Particle Size: 36-44 μm
• Ligand: Phenyl
• Ligand density: Approximately 9 μmol/mL medium
• Dynamic binding capacity: 19 mg BSA/mL resin
• Recommended flow rate: Up to 220 cm/h in a 1 m diameter column with a bed height of 20 cm at 20°C; measured using process buffers with the same viscosity as water at 300 kPa.
• Recommended column height: 20 cm
• Chemical stability: 1 M NaOH, commonly used aqueous buffers.
• pH working range: 2–13
• CIP stability: 2–14
• Shelf life: Five years
• Storage: 20% Ethanol at 4°C to 30°C
• Cleaning-in-place: General recommendation for CIP and sanitization protocols is to use 1 M NaOH. Use of a water-diluted organic solvent, such as ethanol or isopropanol, can be efficient in breaking strong hydrophobic interactions during CIP.
• Sanitization: General recommendation for CIP and sanitization protocols is to use 1 M NaOH. Use of a water-diluted organic solvent, such as ethanol or isopropanol, can be efficient in breaking strong hydrophobic interactions during CIP.
• Pack size: 25 mL
• BioProcess resin: Yes
• Dimensions: 1 m