Capto S ImpAct resin

• Cat#No#: Ca-445C
• Product Overview: With a high binding capacity and small particle size, Capto S ImpAct is a good choice for reliable and robust polishing steps in an industrial purification process.
• Description: Capto S ImpAct chromatography medium (resin) is a strong cation exchanger. The medium is designed for polishing of monoclonal antibodies (MAbs) and a wide range of other biomolecules. Capto S ImpAct is part of a platform of media based on the Capto product line. The polymeric ligand, in combination with the attributes of the base matrix, gives a cation exchanger with high binding capacity. By combining the rigidity of Capto media with an optimized bead, Capto S ImpAct delivers both good pressure/flow properties and good resolution, even at high sample load. The high binding capacity and resolution, combined with the ability to run at high flow rates and bed heights, increase productivity and flexibility in process design.
• Characteristic: High binding capacity, > 100 mg mAb/mL resin.
  Efficient aggregate removal at high load of monoclonal antibodies.
  High-resolution polishing based on the well-established Capto platform.
  Flexibility of design thanks to a large operational window of flow rates and bed heights for easy optimization and scaling.
  High productivity enabling cost-efficient manufacturing.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Metal ion capacity: 37 to 63 umol (H+)/mL medium
• Matrix: High-flow agarose
• Average particle size: ~50 μm
• Ligand: -SO₃-
• Dynamic binding capacity: >85 mg BSA/mL resin, >90 mg lysozyme/mL resin, >100 mg IgG/ mL resin.
• Recommended flow rate: At least 220 cm/h in a 1 m diameter column with bed height 20 cm at 20°C; measured using process buffers with the same viscosity as water at < 3 bar (0.3 MPa).
• Chemical stability: Commonly used aqueous buffers, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol.
• pH working range: 4–12
• CIP stability: 3–14
• Temperature stability: 4°C to 30°C
• Shelf life: 5 years
• Storage: 0.2 M sodium acetate in 20% ethanol at 4°C to 30°C.
• Cleaning-in-place: Precipitated, hydrophobically bound proteins or lipoproteins: Wash with 1 M NaOH solution with reversed flow direction. Contact time 15 to 30 min. Use 1 M NaOH with 1 M NaCl if the results are not satisfactory with only NaOH.
  Ionically bound proteins: Wash with 0.5 to 2 column volumes of 2 M NaCl with reversed flow direction. Contact time 10 to 15 minutes.
  Lipids and very hydrophobic proteins: Wash with 2 to 4 column volumes of up to 70% ethanol or 30% isopropanol with reversed flow direction. Contact time 1 to 2 h. Alternatively, wash with 2 to 4 column volumes of 0.1 to 0.5% non-ionic detergent with reversed flow direction. Contact time 1 to 2 h.
• Sanitization: To reduce microbial contamination in the packed column, sanitization using 0.5 to 1 M NaOH with a contact time of 1 h is recommended. The CIP protocol for precipitated, hydrophobically bound proteins or lipoproteins sanitizes the medium effectively.
• Pack size: 25 mL
• BioProcess resin: Yes
• Maximum flow velocity: At least 220 cm/h in a 1 m diameter column with bed height 20 cm at 20°C; measured using process buffers with the same viscosity as water at < 3 bar (0.3 MPa).
• Functional group: SO3 -