Capto S resin

• Cat#No#: Ca-446C
• Product Overview: Capto S is a strong cation exchanger for fast, efficient, and cost-effective capture and intermediate protein purification from large feed volumes.
• Description: Capto S is strong cation, strong anion, and weak anion exchange resins for packed bed chromatography that increase speed and throughput in capture and intermediate purification. They combine high capacity with high flow velocity and low back pressure to reduce process cycle times and increase productivity.
• Characteristic: Strong cation exchanger for high-productivity capture and intermediate purification when high volume throughput is essential.
  High dynamic binding capacity at high flow raises productivity.
  High-volume throughput cuts process times.
  Cost-effective processing with smaller unit operations.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Maximum operating pressure: 300 kPa at 700 cm/h, 1 m diameter column.
• Ligand Coupling Method: Dextran surface extenders
• Metal ion capacity: 0.11-0.14 mmol Na+/ml medium
• Matrix: Highly cross-linked agarose with dextran surface extender.
• Ionic Exchanger Type: Strong cation exchanger
• Average particle size: ~90 μm
• Ligand: Sulfonate group
• Dynamic binding capacity: > 120 mg lysozyme/mL resin
• Recommended column height: 20 cm
• Chemical stability: Commonly used aqueous buffers, 1 M acetic acid, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol.
• pH working range: 4–12
• CIP stability: 3–14
• Temperature stability: 4°C to 30°C
• Storage: 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate.
• Cleaning-in-place: Precipitated, hydrophobically bound proteins or lipoproteins: Wash with 1.0 M NaOH at 40 cm/h with reversed flow direction. Contact time 1 to 4 h, dependent on feed.
  Ionically bound proteins: Wash with 0.5 to 2 column volumes (CV) of 2 M NaCl with reversed flow direction. Contact time 10 to 15 min.
  Lipids and very hydrophobic proteins: Wash with 2 to 4 CV of up to 70% ethanol1 or 30% isopropanol with reversed flow direction. Contact time 1 to 2 h, dependent on feed. Alternatively, wash with 2 to 4 CV of 0.1% nonionic detergent with reversed flow direction. Contact time 1 to 2 h, dependent on feed.
• Sanitization: To reduce microbial contamination in the packed column, sanitization using 0.5 to 1.0 M NaOH with a contact time of 1 h is recommended.
• Pack size: 25 mL
• BioProcess resin: Yes
• Maximum flow velocity: 700 cm/h
• Functional group: SO3 -