Capto SP ImpRes in ReadyToProcess columns

• Cat#No#: Ca-199P
• Product Overview: These ReadyToProcess chromatography columns are prepacked with Capto SP ImpRes resin and are a cost-effective solutions for intermediate and polishing purification steps. ReadyToProcess columns are validated high-performance bioprocessing columns that are supplied prepacked and ready for use.
• Description: Capto SP ImpRes is strong cation exchangers for the high-throughput intermediate purification and polishing steps of a wide range of biomolecules. Both chromatography media (resins) are part of platform of high-resolution media based on the Capto product line.
  By combining the high-flow characteristics of Capto media with a small particle size, Capto SP ImpRes delivers excellent pressure-flow properties with excellent resolution. The ability to run at higher flow rates and higher bed heights increases productivity and flexibility in process design.
• Characteristic: High-resolution intermediate purification and polishing resin based on the well-established Capto platform with traditional ligands. Flexible process design due to large operational window of flow rates and bed heights.
  High manufacturing productivity enables cost-effective processes.
  BioProcess resin developed for large-scale chromatography.
  Security of supply and comprehensive regulatory support.
  High throughput purifications easy to optimize and scale.
• Applications: For manufacturing of biopharmaceuticals for clinical phase I and II studies. Depending on the scale of operations, they can also be used for full-scale manufacturing, as well as for preclinical studies.
• Maximum operating pressure: 3 bar [0.3 MPa] (44 psi)
• Metal ion capacity: 0.13 to 0.16 mmol (H+ )/mL medium
• Matrix: High-flow agarose
• Average particle size: 40 μm
• Dynamic binding capacity: At least 220 cm/h in a 1 m diameter column with bed height 20 cm at 20°C; measured using process buffers with the same viscosity as water at < 3 bar (0.3 MPa, 43.5 psi).
• Recommended flow rate: > 70 mg lysozyme/mL medium, > 95 mg BSA/mL medium
• Recommended column height: 200 mm (7.87 in)
• Chemical stability: All commonly used aqueous buffers, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
• pH working range: 4 to 12
• CIP stability: 3 to 14
• Storage: 4°C-30°C, 20% EtOH + 0.2 M Sodium Acetate
• Pack size: 1 L
• Maximum flow velocity: 300 cm/h (2.3 mL/min)
• Dimensions: 80 × 200 mm
• Column volume: 1 L
• Column i.d.: 80 mm
• Column hardware pressure limit: 5 bar (0.5 MPa, 70 psi)
• Functional group: –CH2 CH2 CH2 SO3-